SARS-CoV-2 infection after vaccination in Italian health care workers: a case report.
PMID: 35283546
2022
National Academy science letters. National Academy of Sciences, India
Abstract: Their genotyping performed on RNA extracts highlighted the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) sequence variants, all indicative of VOC 202012/01-lineage B.1.1.7, suggesting a common source of infection.
Result: The genotyping performed first by Real-time PCR and then confirmed by direct sequencing proved the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) variants, indicative of VOC 202,012/01-lineage B.
Figure: Sections from the electropherograms showing the 69/70, N501Y, A570D, and D614G (a., b., c., d.) associated with SARS-CoV-2 Alfa Variant B.1.1.7.
Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections.
Discussion: Further follow-up will be needed to confirm whether the specific Nab titer against D614G is maintained or not.
Discussion: The neutralizing titer against D614G significantly decreased in sera of 6-8 months post onset compared to those of 1-3 months post onset ( Figure 3B ).
Discussion: The purpose of this study was to examine the longevity of Nab activity of COVID-19 convalescent sera against D614G, and their neutralizing breadth against B.1.1.7, P.1, and B.1.351.
Discussion: These observations suggested that all participants in this study were likely to be infected with D614G.
Discussion: This reason might be that weak immune-response against SARS-CoV-2 lead to the low Nab titers of 'patients without pneumonia' against PMID: 35279013
2022
Biomedicine & pharmacotherapy
Discussion: Beta variant contains nine mutations in SARS-CoV-2 spike protein: D614G, Delta242-Delta244, R246I, K417N, E484K, N501Y, and A701V.
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Introduction: Experimental data from human lung epithelium and animal models revealed that the D614G substitution increased virus infectivity and transmissibility as compared to an original D614 strain.
Introduction: From January 2020, viruses carrying the spike D614G mutation emerged in several countries.
Introduction: In June, D614G SARS-CoV-2 lineage B.1 became the dominant form of circulating virus worldwide and replaced the initial SARS-CoV-2 strains related to the outbreak in Wuhan, China.
Method: To quantify D614:G614 ratios using a previous
Discussion: Some recent studies regarding transmissibility of Alpha variant or Alpha-like viruses showed a fitness advantage of these viruses on strains carrying D614G spike mutation in the hamster model.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.
Abstract: To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, S371L, S373P, S375F, K417N,
Introduction: Note that we use a polymutant nomenclature without the ending amino acid designated, e.g., D614 as opposed to D614G, because several polymutants exhibit multiple mutations.
Introduction: Some, like D614G, are observed in all three variant families and the impact of this mutation has been well documented.
Variants of SARS CoV-2: mutations, transmissibility, virulence, drug resistance, and antibody/vaccine sensitivity.
PMID: 35227008
2022
Frontiers in bioscience (Landmark edition)
Abstract: Mutations in the S protein that are common among several of the variants include D614G that increases transmissibility and viral load and is often associated with P323L on the RNA dependent RNA polymerase.
In-silico genomic landscape characterization and evolution of SARS-CoV-2 variants isolated in India shows significant drift with high frequency of mutations.
PMID: 35233173
2022
Saudi journal of biological sciences
Abstract: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene (structural gene) at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G).
Result: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G)) Table 3 and.
Table: D614G
Discussion: A23403G (D614G) was observed in West European states in the early stage of the disease and this finding might point towards the source of infection from which it was introduced in India.
Discussion: Moreover, the present study showed that
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Discussion: Here, we showed preservation of Fc effector function against VOCs both in individuals previously infected with the original D614G variant and in individuals vaccinated with Ad26.COV2.S.
Discussion: This is supported by a recent study measuring natural killer (NK) activation, ADCP, ADCD, and ADCC in Ad26.COV2.S vaccinees showing differences of under 2-fold between original D614G and Beta.
Discussion: This was despite the fact that the sequence of immunodominant regions of the eliciting immunogens were the same, with only the single D614G mutation differing between them.
Discussion: We also show subtle differences in the ability of antibodies elicited by either the original D614G or the Ad26.COV2.S vaccine to perform ADCC a
Molecular and Epidemiological Characterization of Emerging Immune-Escape Variants of SARS-CoV-2.
Method: For instance, a Delta variant spike haplotype consisting of T19R, 256_258delinsG,
Result: At 1 week after vaccination, strong neutralization (hiVNT score > 70) of all variants was observed in most of the sera samples, ranging from the highest (95.2%) in D614G to the lowest in the Beta variant (70.6 %) (Figure 3).
Result: The geometric mean titers (GMTs) were 225 for D614G, 38 for Beta, and 37 for Delta + E484K + N501Y (Figure 2A), suggesting that the sera had 6-fold reduced neutralization efficacy against the Beta and Delta variants.
Figure: Since these nAbs are treated as a cocktail, they are considered effective if the EC50 of either antibody is equivalent to or lower than that of the D614G control.
SARS_CoV_2 mutation literature information.
PMID: 
2022
National Academy science letters. National Academy of Sciences, India
Browser Board
Co-occurred Entities
Filtrator
ViMRT