Introduction: The first mutation known to improve viral fitness was Spike D614G, which was first identified in the Alpha variant and then reached a prevalence of nearly 100% globally.
Allosteric Determinants of the SARS-CoV-2 Spike Protein Binding with Nanobodies: Examining Mechanisms of Mutational Escape and Sensitivity of the Omicron Variant.
PMID: 35216287
2022
International journal of molecular sciences
Introduction: The detection of common mutational changes such as D614G, E484K, N501Y, and K417N that are shared among major circulating variants B.1.1.7, B.1.351, and B.1.1.28/P.1 indicated that these positions could be particularly critical for modulation of the SARS-CoV-2 S protein responses.
Introduction: The emergence of variants of concern (VOCs) with the enhanced transmissibility and infectivity profile including the D614G variant, B.1.1.7 (alpha), B.1.351 (beta), B.1.1.28/P.1 (gamma), and B.1.1.427/B.1.429 (epsilon) variants have attracted enormous attention in the scientific community and a considerable variety of the proposed mechanisms explaining functional observations from structural and biochemical perspectives.
Introduction: Using data analysis and protein struc
SARS-CoV-2 Omicron Spike recognition by plasma from individuals receiving BNT162b2 mRNA vaccination with a 16-week interval between doses.
Introduction: Omicron Spike was recognized less efficiently than D614G, Alpha, Beta, Gamma, and Delta Spikes.
Introduction: Plasma from vaccinated previously infected individuals recognized more efficiently all tested Spikes (D614G, Alpha, Beta, Gamma, Delta, and Omicron) than those from naive vaccinated individuals.
Method: The plasmids encoding the SARS-CoV-2 Spike variants; D614G, B.1.1.7, B.1.351, P.1 and B.1.617.2 were previously described.
Method: To produce SARS-CoV-2 pseudoviruses, 293T cells were transfected with the lentiviral vector pNL4.3 R-E- Luc (NIH AIDS Reagent Program) and a plasmid encoding for the indicated S glycoprotein (D614G, Alpha, Beta, Gam
Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction.
PMID: 35219552
2022
Diagnostic microbiology and infectious disease
Table: D614G
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Abstract: Simultaneously we have discussed the significant mutations related to emerging variants and immune escape, such as mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other parts (D614G, P681R) of the S-glycoprotein.
Introduction: Another mutation in the S-glycoprotein region is D614G, which is associated with immune escape.
Introduction: Few mutations include K417T/N, E484K, L452R,
Table: D614G
Molecular and Epidemiological Characterization of Emerging Immune-Escape Variants of SARS-CoV-2.
Method: For instance, a Delta variant spike haplotype consisting of T19R, 256_258delinsG, L452R, T478K, D614G, P681R, and D950N is also assigned to another haplotype group of T19R, L452R, T478K, D614G, P681R, and D950N, which is missing a 256_258delinsG variant.
Result: At 1 week after vaccination, strong neutralization (hiVNT
Figure: Since these nAbs are treated as a cocktail, they are considered effective if the EC50 of either antibody is equivalent to or lower than that of the D614G control.
Variants of SARS CoV-2: mutations, transmissibility, virulence, drug resistance, and antibody/vaccine sensitivity.
PMID: 35227008
2022
Frontiers in bioscience (Landmark edition)
Abstract: Mutations in the S protein that are common among several of the variants include D614G that increases transmissibility and viral load and is often associated with P323L on the RNA dependent RNA polymerase.
In-silico genomic landscape characterization and evolution of SARS-CoV-2 variants isolated in India shows significant drift with high frequency of mutations.
PMID: 35233173
2022
Saudi journal of biological sciences
Abstract: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene (structural gene) at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G).
Result: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G)) Table 3 and.
Table: D614G
Discussion: A23403G (D614G) was observed in West European states in the early stage of the disease and this finding might point towards the source of infection from which it was introduced in India.
Discussion: Moreover, the present study showed that
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
PMID: 35257681
2022
Journal of virological methods
Introduction: For the simultaneous detection of SARS-CoV-2 and its variant (D614G mutant), FAM and ROX fluorophores were attached to each probe during probe sequence design to enable target discrimination.
Introduction: In the experiment, the specificity and sensitivity of RdRp and N target primer pairs and probe sets were confirmed in the L clade, chosen to represent viruses containing the target gene without the D614G mutation.
Introduction: In this milieu, we developed a rRT-PCR detection method that simultaneously detects common SARS-CoV-2 and the SARS-CoV-2 D614 strain (a mutant with the replacement of aspartic acid with glycine at position 614 of the spike glycoprotein (S protein)), which has become a major circulating strain worldwid
SARS_CoV_2 mutation literature information.
PMID: 
2022
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