Figure: (A) In this alpha-complementation assay, 293T target cells expressing ACE2 only or ACE2+TMPRSS2 were incubated with ilomastat and marimastat at the indicated concentration for 2 h before cocultivation with effector cells expressing wild-type or D614G S gp for 4 h.
Figure: (A) Lentivirus particles pseudotyped with the wild-type or D614G S glycoproteins were incubated with various concentrations (0 to 300 nM) of soluble ACE2 (sACE2) for 1 h on ice.
Figure: (B) Lentivirus particles pseudotyped with the wild-type or D614G glycoproteins were incubated with the indicated concentrations of sACE2 for 1 h at 37 C.
Figure: (C and D) VSV vectors pseudotyped with the wild-type, FurinMut, or D614G S gp variants were used to
An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2.
Discussion: SARS-CoV-2 encoding the D614G spike protein has increased its frequency in the human population.
Discussion: The D614G spike protein was found to be more resistant to shedding from the virion, adopting a conformation that favors ACE2 binding and lowers the energy barrier to cell fusion.
Discussion: The ACE2 microbody maintained its ability to neutralize D614G spike protein pseudotyped virus and was able to neutralize diverse beta coronaviruses.
Discussion: The microbody was fully active against virus with the D614G spike protein, a variant of increasing prevalence with increased infectivity in vitro (Figure 6B), and was highly active against ACE2-specific COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Introduction: At the same time, it has been reported that D614G mutation co-occurs with three more mutations, i.e., 241 in UTR, 3307, and 14408.
Introduction: Moreover, we have analyzed the D614G mutations in the samples to obtain information on its evolution in Indian population.
Introduction: Protein-modeling analysis of D614G mutation was carried out to identify the impact on structural changes at protein levels.
Introduction: Recently, a predominant mutation, i.e., D614G in Spike protein, has been identified in virus strains sequenced from European population.
Introduction: There are several reports depicting that D614G mutation in Spike protein is associated with enhanced infectivity and spread of the virus owing to in
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Result: Although both forms of spike displayed significant co-localization with the lysosomal marker Lamp2, the D614G mutation appeared to induce a shift in spike protein sorting towards the lysosome.
Result: Although the relative amounts of lysosome-localized spike varied significantly from one cell to another in both cell populations, digital image analysis quantified the effect of the D614G mutation as 54% increase in its lysosomal staining (p = 0.00000024; Student's t-test; 2-tailed; two-sample, unequal variance), from an average of 817 +/- 44 (standard error of the mean (s.e.m.)) in Htet1/SW1 cells (n = 34 images) to an average of 1265 +/- 64 (s.e.m) in Htet1/SD614G cells (n = 37 images).
CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19.
Abstract: Finally, we have designed an assay for the detection of the D614G mutation and show that all of the samples isolated in the Chelmsford, Essex area between mid-April and June 2020, have the mutant genotype whereas a sample originating in Australia was inf
Method: In addition, the human SARS-CoV-2 (NC_045512.2) genomic sequence was imported to the Beacon Designer 8.2 qPCR assay design software package and an LNA-based genotyping assay for discriminating the D614G mutation (A to G) was designed.
Method: This first was RNA isolated from cell culture infected with Sars-CoV-2 which was isolated from a clinical sample at Westmead Hospital (New South Wales, Australia); COVID19 was confirmed using N1/N2 and E gene RT-qPCR) and the second, a synthetic control RNA (Twist Biosciences), which were included as wild type controls for D614G genotyping.
Defusing SARS-CoV-2: Emergency Brakes in a Vaccine Failure Scenario.
Abstract: The timing and locus for the therapeutic intervention are dictated by the cell entry mechanism and by the selective advantage of the dominant D614G mutation.
SARS-CoV-2 Spike Alterations Enhance Pseudoparticle Titers and Replication-Competent VSV-SARS-CoV-2 Virus.
Abstract: Additionally, we engineered a replication-competent VSV (rVSV) virus to produce the S-D614G variant with a truncated cytoplasmic tail.
Abstract: While the particles can be used to assess S entry requirements, the rVSV G/SMet1D614G 21 virus has a poor specific infectivity (particle to infectious titer ratio).
Potentially adaptive SARS-CoV-2 mutations discovered with novel spatiotemporal and explainable AI models.
Abstract: Functional predictions from structural analyses indicate that, contrary to previous reports, the Asp614Gly mutation in the spike glycoprotein (S) likely reduced transmission and the subsequent Pro323Leu mutation in the RNA-dependent RNA polymerase led to the precipitous spread of the virus.
Result: (2) An alternative, contrasting, hypothesis is that the Asp614Gly mutation allowed for more efficient host cell entry, but decreased production of virus by the cell and it was only the addition of Pro323Leu nsp12 that enhanced the replication efficiency, resulting in increased production of virus.
Result: A recent paper, suggested that the Asp614Gly mutatio
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Abstract: Dynamic tracking of SARS-CoV-2 showed that the D614G variant became predominant after emergence in Europe and North America, but not in China.
Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein D614G mutation became the predominant globally circulating variant after its emergence in the early coronavirus disease 2019 (COVID-19) pandemic.
Abstract: This study suggests that the dynamics of the SARS-CoV-2 D614G mutation during the early-to-mid pandemic is associated with enhanced transmission efficiency in populations with lower ACE2 expression.
Abstract: This supports the idea that lower ACE2 expression is a driving force in
Design of a companion bioinformatic tool to detect the emergence and geographical distribution of SARS-CoV-2 Spike protein genetic variants.
PMID: 33380328
2020
Journal of translational medicine
Introduction: Nevertheless, some other reports stressed a possible selective pressure on the D614G variant, the only frequent variation of the spike protein, recently providing in-vivo evidence of its increased fitness .
Result: Considering the total number of sequences, the most frequent Spike protein variation is confirmed to be D614G.
SARS_CoV_2 mutation literature information.
PMID: 
2020
Journal of virology
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