Method: 293T cells were transfected with pcDNA3.1 plasmids expressing the wild-type or D614G SARS-CoV-2 S glycoproteins, which contain carboxy-terminal His6 tags, using Lipofectamine 3000.
Method: Lentivirus particles containing the wild-type or D614G S gp prepared as described above were used to measure the binding of soluble ACE2 (sACE2) to the viral spike and to study the effect of sACE2 binding on the shedding of the S1 gp from the spike.
Method: To prepare lentivirus (HIV-1) particles containing wild-type or D614G S gp, ~7 x 106 293T cells in T75 flasks were transfected with 7.5 microg of psPAX2 and 7.5 microg of the S-expressing pcDNA3.1 plasmid using Lipofec
An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2.
Introduction: It was active against the D614G variant spike protein and against a panel of beta coronavirus spike proteins and protected human ACE2 transgenic mice against infection.
Result: A variant SARS-CoV-2 containing a D614G point mutation in the spike protein has been found to be circulating in the human population with increasing prevalence.
Result: ACE2 Microbody Blocks Entry of Virus with D614G Spike.
Result: Analysis of the infectivity of the D614G and wild-type pseudotyped viruses on the panel of cell lines showed that the mutation increased the infectivity of virus 2- to 4-fold on 293T, ACE2.293T, Vero, and VeroE6 cells, consistent with previous reports.
Result: Different
Analysis of Indian SARS-CoV-2 Genomes Reveals Prevalence of D614G Mutation in Spike Protein Predicting an Increase in Interaction With TMPRSS2 and Virus Infectivity.
Method: The D614G mutant of the Spike protein was generated by Modeler 9.21.
Result: Among the common variants, the most frequent mutations are 23403 A > G (D614G, S gene), 241 C > T ( Discussion: At the same time, several reports documented that 23,403 A > G (D614G) missense mutation in the Spike protein enhanced the infectivity rate of the virus.
Discussion: We observed in our protein modeling analysis that TMPRSS2 binding to Spike protein is enhanced by this mutation of aspartic acid to glycine (D614G) as it resulted in increased hydrogen bonding interactions.
The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike.
Abstract: The spike D614G mutation increases SARS-CoV-2 infectivity, viral load, and transmission but the molecular mechanism underlying these effects remains unclear.
Abstract: These results are consistent with a lysosomal pathway of coronavirus biogenesis and raise the possibility that a common mechanism may underly the D614G mutation's effects on spike protein trafficking in infected cells and the accelerated entry of SARS-CoV-2 into uninfected cells.
Abstract: We report here that spike is trafficked to lysosomes and that the D614G mutation enhances the lysosomal sorting of spike and the lysosomal accumulation of spike-positive punctae in SARS-CoV-2-infected cells.
Introduction: Here we exten
CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19.
Abstract: Finally, we have designed an assay for the detection of the D614G mutation and show that all of the samples
Result: An analysis of all clinical samples using the D614G genotyping assay revealed that all isolates harboured the A to G transition, characteristic of the more infectious phenotype, whereas the control clinical sample and Twist BioScience control 1 were both wild type, A, at this location (supplementary data file, Table 7).
Discussion: Nonetheless, a SARS-CoV-2 variant carrying the Spike protein amino acid change D614G has replaced the original D614A variant in many locations.
Discussion: These data certainly support the finding that this variant is ubiquitous, as all of the UK isolates tested back as early as 14th April carry the D614G mutation.
Defusing SARS-CoV-2: Emergency Brakes in a Vaccine Failure Scenario.
Abstract: The timing and locus for the therapeutic intervention are dictated by the cell entry mechanism and by the selective advantage of the dominant D614G mutation.
SARS-CoV-2 Spike Alterations Enhance Pseudoparticle Titers and Replication-Competent VSV-SARS-CoV-2 Virus.
Abstract: Additionally, we engineered a replication-competent VSV (rVSV) virus to produce the S-D614G variant with a truncated cytoplasmic tail.
Abstract: While the particles can be used to assess S entry requirements, the rVSV G/SMet1D614G 21 virus has a poor specific infectivity (particle to infectious titer ratio).
Potentially adaptive SARS-CoV-2 mutations discovered with novel spatiotemporal and explainable AI models.
Abstract: Functional predictions from structural analyses indicate that, contrary to previous reports, the Asp614Gly mutation in the spike glycoprotein (S) likely reduced transmission and the subsequent Pro323Leu mutation in the RNA-dependent RNA polymerase led to the precipitous spread of the virus.
Result: (2) An alternative, contrasting, hypothesis is that the Asp614Gly mutation allowed for more efficient host cell entry, but decreased production of virus by the cell and it was only the addition of Pro323Leu nsp12 that enhanced the replication efficiency, resulting in increased production of virus.
Result: A recent paper, suggested that the Asp614Gly mutatio
Impact of Genetic Variability in ACE2 Expression on the Evolutionary Dynamics of SARS-CoV-2 Spike D614G Mutation.
Result: Dynamic Tracking of SARS-CoV-2 S Glycoprotein D614G Mutation.
Result: In addition, the SARS-CoV-2 variant containing the D614G mutation is predominantly transmitted outside of China.
Result: Taken together, we speculate that the lower ACE2 expression in European and North American populations is a result of this genetic variation, and provides the driving force for the positive selection of SARS-CoV-2 S glycoprotein D614G mutation.
Result: The SARS-CoV-2 S glycoprotein D614G mutation may be selected as a result of enhanced transmission ability in populations with lower ACE2 expression (Figure 2).
Result: Therefore, the evolutionary adaptation of the D614G mutation ma
Design of a companion bioinformatic tool to detect the emergence and geographical distribution of SARS-CoV-2 Spike protein genetic variants.
PMID: 33380328
2020
Journal of translational medicine
Introduction: Nevertheless, some other reports stressed a possible selective pressure on the D614G variant, the only frequent variation of the spike protein, recently providing in-vivo evidence of its increased fitness .
Result: Considering the total number of sequences, the most frequent Spike protein variation is confirmed to be D614G.
SARS_CoV_2 mutation literature information.
PMID: 
2020
Journal of virology
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