Introduction: Experimental data from human lung epithelium and animal models revealed that the D614G substitution increased virus infectivity and transmissibility as compared to an original D614 strain.
Introduction: From January 2020, viruses carrying the spike D614G mutation emerged in several countries.
Introduction: In June, D614G SARS-CoV-2 lineage B.1 became the dominant form of circulating virus worldwide and replaced the initial SARS-CoV-2 strains related to the outbreak in Wuhan, China.
Method: To quantify D614:G614 ratios using a previous
Discussion: Some recent studies regarding transmissibility of Alpha variant or Alpha-like viruses showed a fitness advantage of these viruses on strains carrying D614G spike mutation in the hamster model.
A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.
Abstract: In addition, neutralizing activity against the D614G, Alpha and Delta variants was also significantly lower after Beta spike vaccination than after Wuhan spike vaccination.
Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H,
A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.
PMID: 35271889
2022
Journal of virological methods
Result: 94 cases were detected in the CY5.5 channel for the mutant type of D614G and L452R mutations.
Result: Sequencing results overlapped with the results of the Primer-Probe assay, 94 samples had the D614G and L452R mutations with 5 samples encompassing Del69/70.
Result: The 5 samples had detected by the Primer-Probe UK variant had the same melting curves that differed from 89 cases containing D614G, L452R, and T478K mutations.
Result: The same melting curves patterns were detected in all cases for the D614G.
Result: Those samples with negative results for E484K,
Table: D614G
Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.
PMID: 35266951
2022
Genetics and molecular biology
Introduction: Currently, the WHO has identified the Gamma lineage (B.1.1.28.1, P.1 or Gamma; Nextstrain clade 20J/V3) with the following key S mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.
Introduction: Key S mutations: L452R, D614G, P681R, +- (E484Q, Q107H,
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Abstract: Simultaneously we have discussed the significant mutations related to emerging variants and immune escape, such as mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other parts (D614G, P681R) of the S-glycoprotein.
Introduction: Another mutation in the S-glycoprotein region is D614G, which is associated with immune escape.
Introduction: Few mutations include K417T/N, E484K, L452R,
Table: D614G
Molecular and Epidemiological Characterization of Emerging Immune-Escape Variants of SARS-CoV-2.
Method: For instance, a Delta variant spike haplotype consisting of T19R, 256_258delinsG, L452R, T478K, D614G, P681R, and D950N is also assigned to another haplotype group of T19R, L452R, T478K, D614G, P681R, and D950N, which is missing a 256_258delinsG variant.
Result: At 1 week after vaccination, strong neutralization (hiVNT
Figure: Since these nAbs are treated as a cocktail, they are considered effective if the EC50 of either antibody is equivalent to or lower than that of the D614G control.
Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction.
PMID: 35219552
2022
Diagnostic microbiology and infectious disease
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biomedicine & pharmacotherapy
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