Result: The non-synonymous mutations reported for spike protein in Table 2 were distributed as follows: one in the signal peptide (SP), two in the N-terminal domain (NTD), six in the receptor-binding domain (RBD), one downstream of the RBD (D614G), and one in the heptad repeat 1 (HR1) (Refer to.
Discussion: The D614G mutation downstream of the RBD could be associated with higher transmission, pathogenicity, and evasion of immune interventions.
Full-length genome characterization and phylogenetic analysis of SARS-CoV-2 virus strains from Yogyakarta and Central Java, Indonesia.
Discussion: According to phylogenetic tree and sequence distribution analysis, it has been suggested that the dominating D614G globally is caused by a positive selection, while the dominating D614G in Europe is due to a founder effect.
Discussion: An increase in SARS-CoV-2 detection conveys the D614G mutation concurrent with the recent global situation of COVID-19.
Discussion: Further study with a larger sample size and involving risk factors for COVID-19 severity is mandatory to determine the association between the D614G mutation and the severity of COVID-19, particularly in Indonesia.
Discussion: Interestingly, patients infected with SARS-CoV-2 bearing D614G mutations showed
An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants.
Introduction: To what extent this will occur as increasing numbers of people are infected and mount counter immune responses is unknown, but already a variant in the viral spike protein S (D614G) has rapidly emerged from multiple independent events and effects S protein stability and dynamics.
SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma.
Introduction: Four plasma samples did not show neutralization activity against the SARS-CoV-2 WT and SARS-CoV-2 D614G variant.
Introduction: Neutralization activity tested against the SARS-CoV-2 WT and D614G variant also showed variable titers.
Introduction: These antibodies also showed a variable neutralization potency against the SARS-CoV-2 WT and D614G viruses ranging from 3.9 ng/mL to 500.0 ng/mL.
Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation.
Introduction: The capacity for successful adaptation is exemplified by the Spike D614G mutation, that arose in China and rapidly spread worldwide, now accounting for more than 90% of infections.
Method: Amino acid substitutions were introduced into the D614G pCDNA_SARS-CoV-2_Spike plasmid as previously described using the QuikChange Lightening Site-Directed Mutagenesis kit, following the manufacturer's instructions (Agilent Technologies, Inc., Santa Clara, CA).
Result: The infecting strain was assigned to lineage 20B bearing the D614G Spike variant.
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Result: Although the percentage of D614G mutation gradually increased from February towards May in both groups, there was more D614G mutation in severely affected group compared with mildly affected group in March (68.2% vs.
Result: In Table 2, we showed collection period of viral isolates in month and frequency of D614G mutation in mildly affected and severely affected groups.
Result: In the spike protein, 23403A>G mutation at the nucleotide level resulted in D614G mutation at the amino acid level, and it was predominantly found in severely affected group (85.7%) compared with mildly affected group (45.7%, p < 0.001) (Table 1.
Result: Of note, the percentage of D614G mutation was found higher in severely affected group compared with mildly affected group for both
The Novel Coronavirus Enigma: Phylogeny and Analyses of Coevolving Mutations Among the SARS-CoV-2 Viruses Circulating in India.
PMID: 33496683
2020
JMIR bioinformatics and biotechnology
8Discussion: The ""major group"" of SARS-CoV-2 strains (66/95, 69.4%) represents the A2a clade reported previo
Discussion: Few strains from Europe and North America since February 2020 have shown the emergence of mutations like 3037C>T (F106F, NSP3), 23403A>G (D614G), and 28881-28883GGG>AAC (R203K and G204R, N) in the SARS-CoV-2 genome harboring the 14408C>T (P323L) mutation within the RdRP gene, suggesting a probable association or coexistence of 14408C>T (P323L) and the emerging higher number of novel point mutations compared to viral genomes from Asia.
Abstract: Previously reported mutations including D614G in S gene, P4715L in ORF1ab, S194L, R203K, and G204R
Result: D614G mutation in S gene, which corresponds to changes in the spike protein, has already been reported widely in the literature.
Result: A previously reported mutation in S gene - D614G (Aspartate to Glycine), was identified in all the genomes sequenced.
Result: The D614G mutation has been reported to cause a decrease in PCR cycle thresholds, suggestive of higher upper respiratory tract viral load in the host.
SARS_CoV_2 mutation literature information.
PMID: 
2020
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