Result: T205I is a common N gene mutation in global consensus sequences, at 42.9% prevalence, making the co-occurrence of D399N and T205I a relatively rare event at only 0.16% of total T205I mutations.

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Result: Testing of 293T lysates containing N T205I or N T205I/D399N on the Quidel Sofia 2 antigen test confirmed this finding with the antigen test exhibiting an approximately 1000-fold reduced sensitivity between lysates containin

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Result: Whole genome sequencing revealed two coding mutations in the N gene, T205I and D399N.

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