Thermostable designed ankyrin repeat proteins (DARPins) as building blocks for innovative drugs.
PMID: 34793836
2022
The Journal of biological chemistry
Table: D17L
Figure: B, the Asp17Leu mutant (N1C_v05) has a T m of 84.6 C and is strongly stabilized compared with N1C_v01 containing Asp17.
Discussion: Besides the three DARPin domains binding to three unique epitopes of the spike ectodomain of SARS-CoV-2, that all carry Asp17Leu in their N-Caps, ensovibep is in addition composed of two anti-HSA DARPins for serum half-life extension.
Discussion: However, even the investigated DARPin domains aVEGF and aHER2 that possess framework mutations, which are key for their low picomolar binding affinities, still profit from the Asp17Leu mutation.
Discussion: Importantly, the MD simulations show t
Discussion: Quantitative dimension and additive nature of the Asp17Leu improvement.
SARS_CoV_2 mutation literature information.
PMID: 
2022
The Journal of biological chemistry
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