Discussion: Recently, the United Kingdom has reported that a large proportion of new cases in South East England belonged to a new single phylogenetic cluster defined by multiple spike protein mutations (deletions 69-70 and 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H).
Mutations in the B.1.1.7 SARS-CoV-2 Spike Protein Reduce Receptor-Binding Affinity and Induce a Flexible Link to the Fusion Peptide.
Introduction: This variant is characterized by several amino acid deletions and exchanges, with most of the protein-coding mutations found within the surface-anchored spike (S) protein of the virus: del69-70HV, del144Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H (Figure S1a).
Method: These were N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.
Molecular epidemiology of SARS-CoV-2 isolated from COVID-19 family clusters.
Introduction: The B.1.1.7 harbors 17 amino acid changes mostly in the Spike (S) protein (69-70del, 144del, N501Y, A570D, P681H, T716I, S982A, and D1118H).
Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.
PMID: 34146731
2021
Infection, genetics and evolution
Abstract: Structural modeling supported potential functional impact of the D1118H and L452R mutations.
Result: We are not aware of any reports that D1118H impacts transmissibility or morbidity, but the location of this mutation in the Spike protein trimer assembly.
Preliminary Structural Data Revealed That the SARS-CoV-2 B.1.617 Variant's RBD Binds to ACE2 Receptor Stronger Than the Wild Type to Enhance the Infectivity.
Introduction: These mutations in this strain may enhance virus transmissibility and infectivity, including the deletion of residues 69-70 and 144 and the substitution of A570D, D614G, T716I, S982A, D1118H, P681H, K417N, K417T, E484 K and N501Y.
Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.
Method: The mutations (Delta69/70, Delta144, N501Y, A570D, D614G, P681H, S982A, T716I and D1118H or K417N, E484K and N501Y) were introduced by amplification with primers with similar Tm.
Result: In addition to RBD N501Y and NTD DeltaH69/V70, B.1.1.7 is defined by further S mutations across S2 (T716I, S982A, and D1118H) and S1 (DeltaY144, A570D, and
Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species.
Method: The variant B.1.1.7 (GISAID: EPI_ISL_601443) was constructed with total of 9 mutations including 69-70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.
Result: For each sample, a total of nine mutations were found in the spike protein: H69-70-, Y144-, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H.
Discussion: The large number of genomic mutations associated with B.1.1.7, including SNPs (A570D, D614G, P681H, T716I, S982A, and D1118H) highlight the need to rapidly distinguish these subtle mutations in emerging VOCs.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Viruses
Browser Board
Co-occurred Entities
Filtrator
ViMRT