Introduction: Along with it other S protein mutations describing the British variant appeared: HV 69-70 deletion, Y144 deletion, A570D, P681H, T716I, S982A, and D1118H.
E-Volve: understanding the impact of mutations in SARS-CoV-2 variants spike protein on antibodies and ACE2 affinity through patterns of chemical interactions at protein interfaces.
Introduction: The Alpha variant fastly spread across dozens of countries and carried 17 mutations, including eight in the spike protein (HV 69-70 deletion, Y144 deletion, N501Y, A570D, P681H, T761I, S982A, and D1118H), which are the basis of three COVID-19 vaccines licensed.
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.
PMID: 35312321
2022
Journal of chemical information and modeling
Introduction: The S protein mutations in the alpha variant are DeltaH69/DeltaV70, Delta144/144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.
Clinico-Genomic Analysis Reiterates Mild Symptoms Post-vaccination Breakthrough: Should We Focus on Low-Frequency Mutations?
Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.
PMID: 35285246
2022
Applied and environmental microbiology
Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.
Result: To further confirm whether the RT-qPCR results were correct, we conducted NGS analysis to examine eight mutation markers for the Alpha variant (S:Delta69/70,
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Conformational Flexibility and Local Frustration in the Functional States of the SARS-CoV-2 Spike B.1.1.7 and B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism of Functional Dynamics and Protein Stability.
PMID: 35163572
2022
International journal of molecular sciences
Result: In some contrast, mutations in the functional RBD positions (K417, E484) and modifications of mutational variant sites T716I, S982A and D1118H resulted in minor energy changes in both closed (Figure 8A) and open S-B.1.1.7 conformations (Figure 8B).
Result: Indeed, for the S-B.1.1.7 conformations, mutational positions D614G, S982A, D1118H are mostly neutrally frustrated (Figure 7E).
Result: These results are also consistent with the experiments showing that N501Y, T716I and D1118H mutations induce only minimal local conformational changes without affecting stability of the S protein.
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,
SARS_CoV_2 mutation literature information.
PMID: 
2022
MedComm
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