The function of SARS-CoV-2 spike protein is impaired by disulfide-bond disruption with mutation at cysteine-488 and by thiol-reactive N-acetyl-cysteine and glutathione.
PMID: 35123263
2022
Biochemical and biophysical research communications
Abstract: We showed that the substitution of Cys-488 with alanine impaired pseudotyped SARS-CoV-2 infection, syncytium formation, and cell-cell fusion triggered by SARS-CoV-2 spike expression.
Result: Microscopic analysis showed that C488A mutant spike lost syncytium formation and cell-cell fusion activities.
Result: Other spike expressions of the Wuhan-type,
Result: and C488A spike mutant, in which the Cys-488 was substituted with alanine, did not induce syncytium formation.
Result: and the pseudotyped virus infection assay showed that the C488A mutant pseudotyped virus lost apparent infectious activity.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biochemical and biophysical research communications
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