Abstract: Reverse transcription fluorescence resonance energy transfer-polymerase chain reaction (FRET-PCRs) were designed against the two most common mutations in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (A23403G in the spike protein; C14408T in the RNA-dependent RNA polymerase).
Method: Genomic RNA of two SARS-CoV-2 viruses from american type culture collection (ATCC) served as controls and as quantitative standards: 2019-nCOV/USA-WA1/2020 which does not contain the A23403G and the C14408T mutations, and 201/501Y.V1 which contains both mutations.
Method: The 6-carboxyfluorescein (6-FAM)-labeled probes were further designed to contain the unique A23403G or PMID: 34103090
2021
European journal of medical research
Abstract: Studies have demonstrated that the C14408T and A234
Result: Studies have demonstrated that the C14408T and A23403G alterations in the Nsp12 and S proteins are the most prominent alterations in the world, leading to miserable mutations.The spike D614G amino acid change has become the most common variant since December 2019.
Table: C14408T
Discussion: reported C14408T variant on Nsp12 and A23403G variation on S protein, and both cause significant mutations and changes in virus variants worldwide.
Molecular Analysis of SARS-CoV-2 Circulating in Bangladesh during 2020 Revealed Lineage Diversity and Potential Mutations.
Result: Among SARS-CoV-2 sequences reported from Bangladesh, 241C > T, 3037C > T, 14408C > T, 23403A > G, 28881G > A, and 28883G > C mutations were the six most abundant nucleotide mutations that occurred in 98% sequences.
Emerging variants of concern in SARS-CoV-2 membrane protein: a highly conserved target with potential pathological and therapeutic implications.
Abstract: Four genetically linked mutations known as the globally dominant SARS-CoV-2 haplotype (C241T, C3037T, C14408T and A23403G) were found in the majority of consensus sequences.
Result: Four genetically linked mutations previously described as the globally dominant haplotype in April 2020 were found in the majority of our consensus sequences: C241T (100%; 5'UTR region), C3037T (98.6%; silent mutation), C14408T (100%; resulting in P4715L/P323L amino acid change in ORF1ab) and A23403G (100%; resulting in D614G amin
Pan-India novel coronavirus SARS-CoV-2 genomics and global diversity analysis in spike protein.
5Result: Global characterization of the SARS-CoV-2 variants from all ~50,500 viral genomes sequences, suggested three major variants groups as 1,771 isolate Group 1 ""C241T, C
Result: A23403G and C14408T were observed at higher frequencies (>50%) in all the genomes, while G25563T, C13730T, G11083T C6312A, C241T, C3037T, G11083T, C13730T, C28311T, C6312A and C23929T mutations were predominated (>24% frequency) in Indian genomes (Additional File1).
Comparative analysis of mutational hotspots in the spike protein of SARS-CoV-2 isolates from different geographic origins.
Discussion: The D614G is frequently accompanied by a silent mutation 3037C>T and a missense mutation 14408C>T that causes P323L in viral RdRp in mild and severely affected patients.
Genomic Variations in SARS-CoV-2 Genomes From Gujarat: Underlying Role of Variants in Disease Epidemiology.
Figure: The top mutations included C241T, C3037T, C14408T/Pro314Leu, C18877T, A23403G/Asp614Gly, G25563T/Gln57His, and C26735T with frequency >55%.
Discussion: Another mutation, C14408T with a frequency of 96.83%, is present in the Orf1b gene encoding RNA-directed RNA polymerase (RDRP) non-structural protein (nsp12) with a p-value of 0.1440 in deceased versus recovered patients from Gujarat, while also being observed to be statistically significant
AutoVEM: An automated tool to real-time monitor epidemic trends and key mutations in SARS-CoV-2 evolution.
PMID: 33841748
2021
Computational and structural biotechnology journal
Discussion: Moreover, both the single mutation of A23403G or the combined mutations of C241T, C3037T and C14408T could influence the infectivity, pathogenicity or host adaptability of SARS-CoV-2, which further confirmed the 4 specific sites (C241T, C3037T, C14408T and A23403G) were important in the previous H1 haplotype.
Discussion: Our results showed that the early H1 haplotype subgroup with the 4 highly linked sites (C241T, C3037T, C14408T and A23403G) derived H1-2 subgroup had another 5 highly linked sites (T445C,
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Introduction: A total of 607 mutations are reported in RdRp of which 14408C>T (P323L) mutation which lies near the interface domain of RdRp showed highest frequency (10 925 times in 15 140 genotypes) (Table 2).
Introduction: Cluster I includes 3037C>T; NSP3:F106F (non-structural protein3:F106F) and 14408C>T; RdRp:P323L, cluster II includes 3037C>T, 14408C>T and 23403A>G; S:D614G, cluster III includes
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of medical virology
