Abstract: Reverse transcription fluorescence resonance energy transfer-polymerase chain reaction (FRET-PCRs) were designed against the two most common mutations in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (A23403G in the spike protein; C14408T in the RNA-dependent RNA polymerase).
Method: Genomic RNA of two SARS-CoV-2 viruses from american type culture collection (ATCC) served as controls and as quantitative standards: 2019-nCOV/USA-WA1/2020 which does not contain the A23403G and the C14408T mutations, and 201/501Y.V1 which contains both mutations.
Method: The 6-carboxyfluorescein (6-FAM)-labeled probes were further designed to contain the unique A23403G or PMID: 34103090
2021
European journal of medical research
Abstract: Studies have demonstrated that the C14408T and A234
Result: Studies have demonstrated that the C14408T and A23403G alterations in the Nsp12 and S proteins are the most prominent alterations in the world, leading to miserable mutations.The spike D614G amino acid change has become the most common variant since December 2019.
Table: C14408T
Discussion: reported C14408T variant on Nsp12 and A23403G variation on S protein, and both cause significant mutations and changes in virus variants worldwide.
Genomic Epidemiology of SARS-CoV-2 Infection During the Initial Pandemic Wave and Association With Disease Severity.
Result: For example, the four mutations that define OTU_2 (C241T, C30307T, C14408T, and A23403G) rarely had been described separately and similarly with mutations that characterize OTU_3 (G28881A, G28882A, and G28883C; Supplementary Figure S4).
Result: Showing simultaneously four mutations different to OTU_1 (C241T, C3037T, C14408T, and A23403G), OTU_2 is the first group containing the D614G and the P323L mutations in the spike
Comparative analysis of mutational hotspots in the spike protein of SARS-CoV-2 isolates from different geographic origins.
Discussion: The D614G is frequently accompanied by a silent mutation 3037C>T and a missense mutation 14408C>T that causes P323L in viral RdRp in mild and severely affected patients.
Genomic Variations in SARS-CoV-2 Genomes From Gujarat: Underlying Role of Variants in Disease Epidemiology.
Figure: The top mutations included C241T, C3037T, C14408T/Pro314Leu, C18877T, A23403G/Asp614Gly, G25563T/Gln57His, and C26735T with frequency >55%.
Discussion: Another mutation, C14408T with a frequency of 96.83%, is present in the Orf1b gene encoding RNA-directed RNA polymerase (RDRP) non-structural protein (nsp12) with a p-value of 0.1440 in deceased versus recovered patients from Gujarat, while also being observed to be statistically significant
AutoVEM: An automated tool to real-time monitor epidemic trends and key mutations in SARS-CoV-2 evolution.
PMID: 33841748
2021
Computational and structural biotechnology journal
Discussion: Moreover, both the single mutation of A23403G or the combined mutations of C241T, C3037T and C14408T could influence the infectivity, pathogenicity or host adaptability of SARS-CoV-2, which further confirmed the 4 specific sites (C241T, C3037T, C14408T and A23403G) were important in the previous H1 haplotype.
Discussion: Our results showed that the early H1 haplotype subgroup with the 4 highly linked sites (C241T, C3037T, C14408T and A23403G) derived H1-2 subgroup had another 5 highly linked sites (T445C,
Emerging variants of concern in SARS-CoV-2 membrane protein: a highly conserved target with potential pathological and therapeutic implications.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of medical virology
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