Method: SARS-CoV-2-pseudotyped lentiviruses were prepared by co-transfecting the HEK 293T cell line with either the SARS-CoV-2 original spike (D614G) or the SARS-CoV-2 beta spike (L18F, D80A, D215G, K417N, E484K, N501Y, D614G, A701V, 242-244 del) plasmids in conjunction with a firefly luciferase encoding pNL4 lentivirus backbone plasmid.
Genomic surveillance reveals the detection of SARS-CoV-2 delta, beta, and gamma VOCs during the third wave in Pakistan.
Result: The mutational analysis revealed that all the beta variant isolates reported significant and lineage defining mutations as: ORF1a:K1655N (NSP3: K837N) (5230 G>T), S:D80A (21801 A>C), S:D215G (22206 A>G), S:K417N (22813 G>T), S:E484K (23012 G>A), S:N501Y (23063 A>T),
Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity.
PMID: 34722330
2021
Frontiers in cellular and infection microbiology
Result: In contrast, T716I, A570D, D118H and A701V mutations caused a modest reduction in viral infectivity.
Discussion: Pseudovirion with HV69-70 deletion could enhance the infectivity of the virus, while single-site mutation T716I, A570D, D118H, and A701V caused a modest reduction in viral infectivity.
CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern.
Method: The SARS-CoV-2 target sequences include (i) the wild-type (WT) gene fragment of S protein (S; nucleotides [nt] 21,563 to 25,384; GenBank accession number MN908947); (ii) the mutant gene fragments of S protein, including mutations L5F, D80A, D215G, R246I, K417N, L452R/Q, Y453F, T478K, E484Q/K, N501Y, A570D, D614G, P681H, A701V, T716I,
Emergence of B.1.524(G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave.
Abstract: The new B.1.524(G) carried a set of genetic variations, including A701V (position variant frequency = 0.0007) in Spike protein and a novel G114T mutation at the 5'UTR.
Result: Among the nine variations, six (C6312T, C8637T, A10124G, C17518T, C23664T, and C28854T) caused amino acid substitutions (nsp3-T1198I, nsp4-T28I, nsp5-T24A, nsp13-L
Result: African and Brazilian super spreaders, although the E: P71L and S: A701V mutations defining the S.
Result: Those shared by at least 100 genomes were at 13 genome positions (241, 3037, 6312, 8637, 10124, 14408, 17518, 21516, 21622, 23403, 23664, 28133, and 28854) with eight non-synonymous mutations in the ORF1ab (T2016I, T2791I, T3287A, P4715L, L5752F), S gene (D614G, A701V) and the N gene (S194L) respectively.
Method: They are: Result: The B.1.351 control showed mutation in E484K, D614G, A701V, K417N, N501Y, and L242_244L, B.1.1.7 showed mutations in D614G, delH69V70, N501Y, and Q27stop, the control B.1.617.1 showed mutation in E484Q, P681R, L452R, D614G, and P.1 showed mutations in E484K, D614G, K417T, N501Y, and T20N.
Table: A701V
Changing predominant SARS-CoV-2 lineages drives successive COVID-19 waves in Malaysia, February 2020 to March 2021.
Abstract: It is due to lineage B.1.524 viruses containing spike mutations D614G and A701V.
Result: Nonsynonymous mutations seen in the B.1.524 lineage sequences were nsp3-T1198I, nsp4-T28I, nsp5-T24A, nsp12-P323L, nsp13-L428F, spike-D614G, spike-A701V, and N-S194L.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in medicine
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