Introduction: There are seven substitutions (N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H) and three deletions (H69Del, V70Del, and Y144Del) in the spike of the N501Y.V1 variant comparing with the Wuhan-Hu-1 strain (wide type), with N501Y the only mutation in the ACE2 interface of the receptor binding domain (RBD).
The Impact on Infectivity and Neutralization Efficiency of SARS-CoV-2 Lineage B.1.351 Pseudovirus.
Introduction: In the case of B.1.1.7, a series of mutations in eight sites including D614G appeared in the spike protein: Delta69Delta70, Delta144Delta145, N501Y, A570D, P681H, T716I, S982A, and D1118H (; GISAID, accessed on 1 December 2020).
Dieckol and Its Derivatives as Potential Inhibitors of SARS-CoV-2 Spike Protein (UK Strain: VUI 202012/01): A Computational Study.
Introduction: The new variant is characterized by many mutations such as Delta69-70, Delta144, Tyr453Phe, Asn501Tyr, Ala570Asp, Asp614Gly, Phe681His, Thr716Ile, Ser982Ala, and Asp1118His in the spike protein.
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Result: In addition, the UK-VOC contains several nonsynonymous mutations that cause seven aa substitutions at positions N501Y, A570D, D614G, P681H, T716I, S982A, D1118H in S-protein, in which N501Y mutation occurs in the key residue of RBD.
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Introduction: In addition to these mutations, A570D (RBD), Delta144/145 (S1 subunit), T716I, S982A and D1118H (S2 subunit) are also reported in VUI202012/01.
Table: A570D
Structural Consequences of Variation in SARS-CoV-2 B.1.1.7.
PMID: 33969357
2021
Journal of cellular immunology
Abstract: The mutations at A570D, D614G and S982A reduced contact between individual chains of the trimeric spike protomer, potentially enhancing cleavage into S1 and S2 subunits, dynamic structural rearrangement and host cell fusion mechanisms.
Result: Collectively, these data suggest that: 1) the UK variant B.1.1.7 exhibits a change that enhances affinity for the coronavirus receptor ACE2 (N501Y), and 2) mutations may enhance dynamic virus fusion mechanisms by reducing intermolecular stability of spike protein subunits (A570D, D614G, S982A).
Result: The A570D substitution in the UK variant B.1.1.7 variant introduces steric clash with
A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern.
PMID: 33975021
2021
Infection, genetics and evolution
Abstract: Here we propose a rapid and accessible protocol based on Sanger sequencing of a single PCR fragment that is able to identify and discriminate all SARS-CoV-2 variants of concern (VOCs) identified so far, according to each characteristic mutational profile at the Spike-RBD region (K417N/T, E484K, N501Y, A570D).
Will Mutations in the Spike Protein of SARS-CoV-2 Lead to the Failure of COVID-19 Vaccines?
PMID: 33975397
2021
Journal of Korean medical science
Introduction: The UK SARS-CoV-2 B.1.1.7 variant is defined by multiple spike (S) protein changes (deletion 69-70, deletion 145, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H).
Implications of the Novel Mutations in the SARS-CoV-2 Genome for Transmission, Disease Severity, and the Vaccine Development.
Introduction: The B.1.1.7 variant-specific non-synonymous mutations and deletions have been detected in the spike protein including deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H.
Convalescent-Phase Sera and Vaccine-Elicited Antibodies Largely Maintain Neutralizing Titer against Global SARS-CoV-2 Variant Spikes.
Introduction: The B.1.1.7 lineage (VOC-202012/01) variant identified in patients in the United Kingdom encodes a spike protein with 8 mutations in addition to D614G (Delta69-70, Y144Del, N501Y, A570D, P681H, T716I, S982A, and D1118H).
Result: Analysis of the B.1.1.7 variant and its component mutations showed that the single point mutations had little effect on infectivity (Delta69-70, Y144Del, N501Y, A570D, P681H, and D1118H), except for T716I, which had 5.8-fold decreased infectivity, and S982A, whi
SARS_CoV_2 mutation literature information.
PMID: 
2021
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