Abstract: Apart from that the group of SNVs became dominant, which is represented by two nonsynonymous mutations A23403G (S:D614G) and C14408T (ORF1ab:P4715L), a few emerging groups of SNVs were recognized with sharply increased monthly incidence ratios of up to 70% in November 2020.
Introduction: The majority of SARS-CoV-2 genomes have evolved with a dominant SNV cluster represented by nonsynonymous mutations Result: It is not surprising to see that group A of SNVs has prevailed since June 2020, represented by A23403G (S:D614G) and C14408T (OFR1ab:P4715L).
Introduction and Characteristics of SARS-CoV-2 in North-East of Romania During the First COVID-19 Outbreak.
Discussion: Specifically, mutations at positions 241 (non-coding), 3037 C > T, 14408 C > T, 20268 20003A > G, and 23403 A > G are frequent in European samples and were identified early in the pandemic evolution, as a signature for one of the superspreaders that originated from Wuhan.
Discussion: This mutation co-evolved with other three major mutations, 3037 C > T, 14408 C > T, and 23403 A > G.
Rapid, inexpensive methods for exploring SARS CoV-2 D614G mutation.
Abstract: A common mutation has occurred in the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), known as D614G (A23403G).
Method: Forward and reverse primers (D614G IN F and D614G IN R), designed to cover both sides of the Discussion: Additionally, the researchers should be reminded that our methods are actually testing for the A23403G nucleotide mutation, which mostly encodes the D614G amino acid mutation.
Discussion: By 12 February 2021, the D614G (A23403G) is the most prevalent single nucleotide variations (SNVs) in the world that is about 439,559 sequences (95.5%).
In-Silico analysis reveals lower transcription efficiency of C241T variant of SARS-CoV-2 with host replication factors MADP1 and hnRNP-1.
PMID: 34307830
2021
Informatics in medicine unlocked
Introduction: Top variants in the SARS-CoV-2 genome include high-frequency variants including C241T, C1059T, C3037T, C14408T, A23403G, G25563T, and G28883C, out of which the C241T variant had a 99 % frequency with 0.505 entropy by October 2020.
Near-Complete Genome Sequences of Nine SARS-CoV-2 Strains Harboring the D614G Mutation in Malaysia.
Result: There was only one single nucleotide difference of crRNA-0 at 23403 (A>G)
Figure: Synthetic mismatches are placed in -3 to +3 position of the SNP (A23403G) site of D614G mutation.
Figure: The 3' end of the forward primer is located at the SNP (A23403G) site of D614G mutation with a synthetic mismatch which is the same as that of crRNA-1.
Discussion: Therefore, we designed crRNAs with additional nucleotide mismatches from the -3 to +3 positions centered around the A23403G site.
Discussion: When coupled with a synthetic mismatch primer, whose 3' end is located at the D614G SNP (A23403G) site, the D614G mutation was selectively amplified.
Mutation hotspots and spatiotemporal distribution of SARS-CoV-2 lineages in Brazil, February 2020-2021.
Result: Three mutations were found in > 95% of the genomes: A23403G (S:D614G), C14408T (ORF1ab:L4715), and C3037T (ORF1ab:F924), which are signatures of the B.1 and derived lineages that spread early in the pandemic.
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Method: The five missense mutations, D614G (23403A>G)-S gene, Q57H (25563G>T)-ORF3a gene, P323L (14408C>T):ORF1ab/RdRP gene, R203K (28881G>A):N gene, and G204R (28883G>C):N gene (Table S2).
Result: According to the % frequencies of the genetic markers A570D (23271C>A), D614G (23403A>G), P681H (23604C>A), T716I (23709C>T
Emergence and spread of the potential variant of interest (VOI) B.1.1.519 of SARS-CoV-2 predominantly present in Mexico.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of medical virology
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