Result: Interestingly, we found that the A23403G mutation fine-tuned the two local bulge structures into a thermodynamically more favorable six-nucleotide bulge structure (-10.3 kcal/mol vs.
Figure: The C241U, C3037U, C14408U, and A23403G (D614G) mutants are marked as solid lines.
SARS-CoV-2: Origin, Evolution, and Targeting Inhibition.
PMID: 34222046
2021
Frontiers in cellular and infection microbiology
Introduction: As for the A23403G, it encodes the mutant D614G in Spike protein.
Introduction: In these SNPs, four SNPs, C3037U, C14408U, A23403G, and C241U, show high frequency.
Genomic monitoring unveil the early detection of the SARS-CoV-2 B.1.351 (beta) variant (20H/501Y.V2) in Brazil.
Abstract: Apart from that the group of SNVs became dominant, which is represented by two nonsynonymous mutations A23403G (S:D614G) and C14408T (ORF1ab:P4715L), a few emerging groups of SNVs were recognized with sharply increased monthly incidence ratios of up to 70% in November 2020.
Introduction: The majority of SARS-CoV-2 genomes have evolved with a dominant SNV cluster represented by nonsynonymous mutations Result: It is not surprising to see that group A of SNVs has prevailed since June 2020, represented by A23403G (S:D614G) and C14408T (OFR1ab:P4715L).
Genomic Epidemiology of SARS-CoV-2 Infection During the Initial Pandemic Wave and Association With Disease Severity.
Abstract: Conclusions and Relevance: Within weeks of SARS-CoV-2 circulation, a profound shift toward 23403A>G (D614G) specific genotypes occurred.
Abstract: Infection by strains lacking the 23403A>G variant showed higher mortality in multivariable analysis (odds ratio [OR], 22.4; 95% CI, 0.6 to 5.6; P = .01).
Abstract: Several variants were associated with lower hospitalization rate, and those containing 23403A>G (D614G Spike) were associated with increased survival when the patient was hospitalized (64 of 74 patients [86.5%] vs 10 of 17 patients [58.8%]; chi21 = 6.907; P = .009).
Introduction: Three clades (G, GH, and GR) contain the 23403A>G (D614G) variant within the gene that enc
Mutation hotspots and spatiotemporal distribution of SARS-CoV-2 lineages in Brazil, February 2020-2021.
Result: Three mutations were found in > 95% of the genomes: A23403G (S:D614G), C14408T (ORF1ab:L4715), and C3037T (ORF1ab:F924), which are signatures of the B.1 and derived lineages that spread early in the pandemic.
dsmCRISPR: Dual synthetic mismatches CRISPR/Cas12a-based detection of SARS-CoV-2 D614G mutation.
Result: There was only one single nucleotide difference of crRNA-0 at 23403 (A>G)
Figure: Synthetic mismatches are placed in -3 to +3 position of the SNP (A23403G) site of D614G mutation.
Figure: The 3' end of the forward primer is located at the SNP (A23403G) site of D614G mutation with a synthetic mismatch which is the same as that of crRNA-1.
Discussion: Therefore, we designed crRNAs with additional nucleotide mismatches from the -3 to +3 positions centered around the A23403G site.
Discussion: When coupled with a synthetic mismatch primer, whose 3' end is located at the D614G SNP (A23403G) site, the D614G mutation was selectively amplified.
Introduction and Characteristics of SARS-CoV-2 in North-East of Romania During the First COVID-19 Outbreak.
Discussion: Specifically, mutations at positions 241 (non-coding), 3037 C > T, 14408 C > T, 20268 20003A > G, and 23403 A > G are frequent in European samples and were identified early in the pandemic evolution, as a signature for one of the superspreaders that originated from Wuhan.
Discussion: This mutation co-evolved with other three major mutations, 3037 C > T, 14408 C > T, and 23403 A > G.
Near-Complete Genome Sequences of Nine SARS-CoV-2 Strains Harboring the D614G Mutation in Malaysia.
Abstract: A common mutation has occurred in the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), known as D614G (A23403G).
Method: Forward and reverse primers (D614G IN F and D614G IN R), designed to cover both sides of the Discussion: Additionally, the researchers should be reminded that our methods are actually testing for the A23403G nucleotide mutation, which mostly encodes the D614G amino acid mutation.
Discussion: By 12 February 2021, the D614G (A23403G) is the most prevalent single nucleotide variations (SNVs) in the world that is about 439,559 sequences (95.5%).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Nature communications
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