Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern.
Abstract: Our assay, compared to Sanger sequencing for single nucleotide polymorphism detection, demonstrated positive percent agreement of 100% for all 57 specimens displaying the N501Y mutation, which were confirmed by Sanger sequencing to be typed as A23063T, including one specimen with mixed signal for wild type and mutant.
Abstract: The N501Y amino acid mutation caused by a single point substitution A23063T in the spike gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is possessed by three variants of concern (VOCs), B.1.1.7, B.1.351, and P.1.
Method: Primers and probes selected for the detection of SNP A23063T are reported in Table S1 in the supplemental material.
Result: All specimens with a k
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2.
PMID: 34656702
2022
Journal of virological methods
Introduction: Two MGB TaqMan probes, with FAM and VIC-labelled respectively, were used to differentiate the nucleotide A from T at position 23063, where the N501Y mutation is located.
SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability.
Introduction: All three VoCs have the mutation A23063T, also named N501Y, in common, which is involved in the receptor-binding mechanism and may have clinical impacts.
Mass Screening of SARS-CoV-2 Variants using Sanger Sequencing Strategy in Hiroshima, Japan.
Method: The initial checkpoint was set at nucleotide position nt23063 and if we found mutation from adenine (A) to thymine (T) at nt23063, further identification was done as follows: double mutation of A23063T (referred to N501Y) and C23271A (referred to A570D) for B.1.1.7 (Alpha), G23013A (referred to E484K) and A23063T for B.1.351 (Beta) and triple mutation of G23012A, A23063T and C23525T (referred to H655Y) for P.1 (Gamma).
Genomic Surveillance of SARS-CoV-2 Lineages Indicates Early Circulation of P.1 (Gamma) Variant of Concern in Southern Brazil.
Result: However, p.Asn501Tyr variant and other 8 of the 17 variants (3267C>T p.Thr1001Ile, 6954T>C p.Ile2230Thr in the ORF1ab gene; 23063A>T, 23271 C>A p.Ala570Asp, 23604C>A p.Pro681His, 23709C>T p.Thr716Ile in the S gene; 27972C>T p.Gln27*, 28048G>T p.Arg52Ile in the Orf8 gene;
Clinico-Genomic Analysis Reiterates Mild Symptoms Post-vaccination Breakthrough: Should We Focus on Low-Frequency Mutations?
Result: Within this clade, 14 of the 16 sequences differed from the primary outbreak strain in Facility A by only the A23063T substitution that yielded the N501Y change.
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbiology spectrum
