Result: However, one out of two birds inoculated with H4N2_T327K had multifocal antigen distribution in the myocardium with mild, acute, focal to oligofocal necrotizing myocarditis as well as focal to oligofocal distribution in neuroglial cells with mild, acute, focal to oligofocal, necrotizing polioencephalitis (Figure 6 and Figure 7).

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Result: In MDCK cells, H4N2 viruses carrying authentic HA, HA_T327R or

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Result: The H4N2_T327K group exhibited moderate virulence with an IVPI of 0.6 and moderate to severe neurological disorders (e.g., torticollis, opisthotonos and paresis) starting at 9 dpi were observed in 7 out of 10 injected chickens.

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