Identification of Important N-Linked Glycosylation Sites in the Hemagglutinin Protein and Their Functional Impact on DC-SIGN Mediated Avian Influenza H5N1 Infection.
PMID: 33451024
2021
International journal of molecular sciences
Result: H5N1-RG viruses carrying N27Q or N39Q or both mutations on HA were generated using site-directed mutagenesis.
Discussion: Further, we used monocyte derived iDCs and mDCs to confirm these findings and found that only N27Q and N39Q<
Discussion: Our results demonstrated that most single mutations on N-linked glycosylation sites on HA5 did not influence the binding between DC-SIGN and HA (H5), whereas only three mutations (N27Q, N39Q, and N181Q) reduced this interaction; suggesting that the DC-SIGN CRD may interact minimally with a N-glycosylation with high-mannose oligosaccharides expression on HA protein.
IV mutation literature information.
PMID: 
2021
International journal of molecular sciences
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