Neddylation of M1 negatively regulates the replication of influenza A virus.
PMID: 33016861
2020
The Journal of general virology
Abstract: However, we found that NEDD8 overexpression can still inhibit the replication of PB2 K699R mutant viruses, implying that other viral protein(s) can be neddylated.
NEDDylation of PB2 Reduces Its Stability and Blocks the Replication of Influenza A Virus.
Result: The level of NEDDylation in the K699R mutant was dramatically reduced compared to that of wild type and other PB2 mutants.
Result: The relative levels of FLAG-PB2 and FLAG-PB2 K699R from 2 independent experiments were quantified.
Result: The total cell lysates were harvested for immunoblotting to determ
Result: We infected MDCK cells with WSN or WSN-PB2 K699R and harvested the supernatants at different time points (6, 12, 24, and 36 h post-infection) for plaque assay.
Result: the level of ubiquitination of PB2-WT and PB2-K699R are comparable, suggesting that K699 is not a ubiquitination site.
IV mutation literature information.
PMID: 
2020
The Journal of general virology
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