Abstract: The structure of the K627E mutated domain shows no structural differences but the charge reversal disrupts a striking basic patch on the domain surface.
Introduction: The high resolution crystal structure of this domain from a human influenza A strain and of the K627E variant, shows that it has a novel fold with Lys/Glu627 exposed to the solvent.
Method: For the very high resolution native 627-domain and K627E structures individual atomic anisotropic B-factors were refined.
Method: The mutation K627E in the 538-693 protein was made by PCR mutagenesis.
Method: The structure of the K627E mutant was obtained by refinement.
Result: The structure of the K627E mutant domain is essentially identical (RM
IV mutation literature information.
PMID: 
2008
PLoS pathogens
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