Bioluminescence-based neuraminidase inhibition assay for monitoring influenza virus drug susceptibility in clinical specimens.
PMID: 23917311
2013
Antimicrobial agents and chemotherapy
Abstract: Notably, the QFlu assay identified oseltamivir-resistant A(H1N1)pdm09 viruses carrying the H275Y marker directly in clinical specimens, which is not feasible with the other two phenotypic assays, which required prior virus culturing in cells.
Abstract: To provide proof of principle, clinical specimens (n = 235) confirmed by real-time reverse transcription (RT)-PCR to contain influenza virus A(H1N1)pdm09 and prescreened for the oseltamivir resistance marker H275Y using pyrosequencing were subsequently tested in the QFlu assay.
[Clinical characteristics and molecular epidemiology of the novel influenza A (H1N1) infection in children in Shanghai].
Abstract: OBJECTIVE: To investigate the epidemiological features, genetic drift in the epitopes of hemagglutinin (HA) of the novel influenza A (H1N1) virus and oseltamivir-resistant variants characterized by H275Y and N295S mutations in children in Shanghai since the outbreak.
Abstract: The H275Y and N295S amino acid mutations associated with oseltamivir resistance were not found in the circulating novel influenza (H1N1) strains.
Detection and management of antiviral resistance for influenza viruses.
PMID: 24215378
2013
Influenza and other respiratory viruses
Abstract: Recent studies have shown that, in the presence of the appropriate permissive mutations, the H275Y variant can retain virulence and transmissibility in some viral backgrounds.
Abstract: The most frequently reported change conferring oseltamivir resistance in that viral context is the H275Y neuraminidase mutation (N1 numbering).
Generation and Characterization of Recombinant Influenza A(H1N1) Viruses Resistant to Neuraminidase Inhibitors.
PMID: 24524021
2013
Osong public health and research perspectives
Introduction: Particularly, most isolated seasonal influenza A(H1N1) viruses during the 2008-2009 season were found to encode the H275Y substitution in the NA gene, conferring resistance to oseltamivir.
Introduction: The dominant change conferring oseltamivir resistance in the current seasonal influenza viruses is a mutation in the NA gene, H275Y (N1 numbering).
Introduction: The frequency of isolates with th
Discussion: A previous study has reported that I117V has a synergistic effect of increasing resistance with H275Y, whereas the I117M mutation does not alter oseltamivir sensitivity in the recombinant viruses with NA from the pandemic (H1N1) 2009 virus and the remaining genes from A/PR/8/34.
Synergistic combinations of favipiravir and oseltamivir against wild-type pandemic and oseltamivir-resistant influenza A virus infections in mice.
Introduction: In addition, these inhibitors were also tested in a newly developed oseltamivir-resistant (H275Y, N1 numbering) virus infection model in mice.
Discussion: Further work with the A/Mississippi/3/2001 (H1N1) H275Y mouse model will continue as novel inhibitors are discovered and used in combination with oseltamivir.
Discussion: In addition, H275Y-carrying viruses that are resistant to oseltamivir were effectively treated in mice with the combination of oseltamivir and favipiravir.
Discussion: In the case of the present research, there is no guarantee that the mouse-adapted wild-type influenza A/California/04/2009 (H1N1pdm) virus can be converted to an H275Y virus by genetic manipulation and still retain its virulence in mice.
Discussion: In the infection of mice with the oseltamivir-resistant influenza A/Mississippi/3
Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus A(H1N1), A(H1N1)2009, and A(H3N2) subtypes by melting point analysis.
PMID: 22089329
2012
European journal of clinical microbiology & infectious diseases
Abstract: We evaluated the use of a procedure involving real-time polymerase chain reaction (PCR) followed by melting point analysis (MPA) of hybrids formed between the PCR product and a specific oligonucleotide probe for the identification of point mutations in the influenza A virus neuraminidase gene (NA) that are associated with oseltamivir resistance [resulting in the amino acid change H275Y for seasonal and pandemic influenza A(H1N1) viruses and E119V for A(H3N2) viruses].
The 2008-2009 H1N1 influenza virus exhibits reduced susceptibility to antibody inhibition: Implications for the prevalence of oseltamivir resistant variant viruses.
Introduction: However, the emergence of a H275Y resistant H1N1 variant in patients without pre-exposure to oseltamivir was identified in Norway in 2007.
Introduction: It was also shown that resistant mutants obtained through selection in culture or isolated from patients treated with neuraminidase inhibitors are subtype specific, with E119V and R292K NA mutations occurring mainly in H3N2 subtype viruses exposed to either oseltamivir or zanamivir, and the H275Y mutation in the NA protein found almost exclusively among H1N1 subtype viruses in response to oseltamivir treatment.
Introduction: Prior to 2007, only a very small number of H275Y mutant oseltamivir resistant viruses were recognized, mainly cl
Impact of mutations at residue I223 of the neuraminidase protein on the resistance profile, replication level, and virulence of the 2009 pandemic influenza virus.
PMID: 22203589
2012
Antimicrobial agents and chemotherapy
Abstract: As expected, the H275Y mutation conferred resistance to oseltamivir (982-fold) and peramivir (661-fold) compared to the drug-susceptible recombinant WT.
Abstract: Consequently, I223R and I223V mutations, alone or with H275Y, need to be thoroughly monitored.
Abstract: Infectivity and transmissibility in ferrets were comparable between the recombinant WT and the H275Y or I223V-H275Y recombinants.
Abstract: Infectivity and transmission of the WT, H275Y, and I223V-H275Y recombinant viruses were evaluated in ferrets.
Abstract: Interestingly, enhanced levels of resistance to oseltamivir and peramivir and reduced
Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y.
PMID: 22243670
2012
Influenza and other respiratory viruses
Abstract: Further investigations are needed to determine the significance of infection with strains possessing NA-H275Y and HA-D222G.
Abstract: Oseltamivir-resistant 2009 H1N1 influenza virus infections associated with neuraminidase (NA) H275Y have been identified sporadically.
Abstract: We report the first clinical description of 2009 H1N1 virus infection with both NA-H275Y and HA-D222G mutations detected by pyrosequencing of bronchioalveolar lavage fluid obtained on
Susceptibility of avian influenza viruses of the N6 subtype to the neuraminidase inhibitor oseltamivir.
Discussion: Interestingly, substitutions of I to R, K or V, at the 222 NA residue of human H1N1 2009 viruses confer moderately reduced susceptibilities to both zanamivir and oseltamivir but, when combined with the H275Y NA substitution, cause even higher levels of resistance to oseltamivir and peramivir than the H275Y substitution alone.
IV mutation literature information.
PMID: 
2013
Antimicrobial agents and chemotherapy
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