Abstract: In a cytopathic effect assay, SA-2 dose dependently inhibited H1N1, H3N2 and the oseltamivir-resistant mutant H1N1-H275Y influenza viruses in both virus-infected MDCK and A549 cells, with 50% effective concentrations (EC50) in MDCK cells of 9.6, 19.2 and 19.8 muM respectively, and 50% cytotoxic concentration (CC50) of 444.5 muM, showing competitive antiviral activity with oseltamivir in vitro.
Abstract: Orally administered SA-2 effectively protected mice infected with lethal doses of H1N1 or oseltamivir-resistant strain H1N1-H275Y, conferring 70% or 50% survival at a dosage of 100 mg/kg/d, reducing body weight loss, alleviating the influenza-induced acute lung injury, and reducing lung virus titer.
Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y.
Abstract: An influenza A(H1N1)pdm09 virus carrying a G147R substitution in combination with an H275Y substitution in the neuraminidase protein, which confers cross-resistance to oseltamivir and peramivir, was detected from an immunocompromised inpatient in Japan, March 2016.
Abstract: This dual H275Y/G147R mutant virus exhibited enhanced cross-resistance to both drugs compared with the single H275Y mutant virus and reduced susceptibility to zanamivir, although it showed normal inhibition by laninamivir.
Mutations at highly conserved residues in influenza A(H1N1)pdm09 virus affect neuraminidase activity.
Abstract: NA mutations N386K and P431S together or separately, and in the presence or absence of H275Y were further evaluated using recombinant influenza A/California/04/2009 (pH1N1) viruses containing single, double, or triple mutations.
Abstract: Viral growth was reduced in the presence of mutation P431S alone or combined with N386K and/or H275Y.
Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient.
PMID: 27849220
2016
Memorias do Instituto Oswaldo Cruz
Discussion: These samples should be analysed immediately using molecular-based techniques for common resistance-associated mutations [e.g., E119V in A(H3N2) and H275Y in A(H1N1)pdm09 viruses], and prompt feedback must be given to treating clinicians, so that alternative therapies can be initiated.
Virological characterization of influenza H1N1pdm09 in Vietnam, 2010-2013.
PMID: 25966032
2015
Influenza and other respiratory viruses
Abstract: One isolate from 2011 and one isolate from 2013 had a predicted H275Y substitution in the neuraminidase molecule, which was associated with reduced susceptibility to oseltamivir in a NAI assay.
Introduction: In addition, substitutions <
Result: A significant mutation at H275Y related to reduced susceptibility to oseltamivir was found in two isolates in 2011 and 2013 located in groups 7A and 5 (Figure4).
Discussion: Two oseltamivir-resistant A/H1N1pdm09 isolates in 2011 and 2013 were detected by neuraminidase inhibition (NAI) test and Sanger sequence NA gene (H275Y); in total, 11 cases of Vietnamese oseltamivir-resistant A/H1N1pdm09 isolates have been identified between 2009 and 2013.
Phylogenetic analysis of the neuraminidase gene of pandemic H1N1 influenza A virus circulating in the South American region.
Abstract: 3.4% of the strains enrolled in these studies carried the H275Y substitution that confers resistance to oseltamivir.
Oseltamivir-resistant influenza A(H1N1)pdm2009 strains found in Brazil are endowed with permissive mutations, which compensate the loss of fitness imposed by antiviral resistance.
PMID: 25742269
2015
Memorias do Instituto Oswaldo Cruz
Abstract: Consequently, OST-resistant strains, carrying the mutation H275Y, emerged in the years after the pandemics, with a prevalence of 1-2%.
Abstract: Importantly, the change D344N, also predicted to compensate loss of fitness imposed by H275Y mutation, was found in Brazil, but not in other countries in 2013.
Abstract: To spread in community settings, H275Y mutants must contain additional mutations, collectively called permissive mutations.
Result: 1) (H275Y viruses in red).
Result: 1) and are predicted to compensate for the negative effects of H275Y change (Hurt et al.
Result: Although low prevalence of H275Y viruses with permissive mutations in the <
Table: H275Y
Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2013-2014.
Introduction: Additional NA substitutions (R222Q, V234M, D344N and D354G) compensated for the detrimental effect of the H275Y substitution on virus fitness, allowing the virus to spread efficiently.
Introduction: Animal models have shown that A(H1N1)pdm09 H275Y viruses with additional NA amino acid substitutions, V241I and N369K, have increased replication and transmission fitness.
Introduction: Despite >99% of circulating viruses being sensitive to all four NAIs during the 2012-2013 period, localised community circulation of influenza viruses with RI or HRI has occurred in recent years, most nota
Identification of novel compounds against an R294K substitution of influenza A (H7N9) virus using ensemble based drug virtual screening.
PMID: 25589893
2015
International journal of medical sciences
3Introduction: Single amino acid change known as the ""H275Y"" mutation in 2009 H1N1 flu virus and ""R292K"" mutation in influenza A virus is conferred by drug resistance."
[Analysis of HA and NA Genes of Influenza A H1N1 Virus in Yunnan Province during 2009-2014].
IV mutation literature information.
PMID: 
2016
Antiviral research
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