Result: Indeed, H84T inhibited replication of A/Mississippi/3/2001-H275Y (H1N1), a strain reported to be oseltamivir-resistant, in Madin-Darby canine kidney (MDCK) cells, with an EC90 value of 0.074 mug/mL (2.4 nM), as compared to >100 mug/mL for oseltamivir.
Result: S1), in which NA inhibitor resistance is conferred by the E119V and H275Y mutations, respectively, and adamantane resistance conferred by the S31N mutation.
Baloxavir for the treatment of Influenza in allogeneic hematopoietic stem cell transplant recipients previously treated with oseltamivir.
Abstract: RESULTS: Two patients were infected with influenza A/H1pdm09 carrying a neuraminidase variant (H275Y) linked to oseltamivir resistance.
Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018-2019 winter season.
PMID: 32459617
2020
Journal of medical microbiology
Method: Screening of A(H1N1)pdm09 viruses for the presence of point mutations encoding NA H275Y amino acid substitution, known to confer oseltamivir resistance, was carried out using a real-time RT-PCR assay that allowed discrimination of a single nucleotide difference between oseltamivir sensitive and resistant viruses.
Result: Testing was performed on 280 detected A(H1N1)pdm09 viruses by real-time RT-PCR with respect to the NA H275Y oseltamivir resistance substitution at the NRL in Bulgaria; NA and PA sequences of all study A(H1N1)pdm09 and A(H3N2) viruses were screened for known markers of reduced susceptibility to NA inhibitors and baloxavir marboxil and phenotypic testing (by the MUNANA method) was performed on 13 influenza A(H1N1)pd
Genetic and antigenic characterization of influenza A/H5N1 viruses isolated from patients in Indonesia, 2008-2015.
Discussion: Despite some studies reporting a statistically significant impairment in viral growth in vitro, other groups' analysis have not identified significant differences regarding the replicative capacity between the pdm09 Wt virus and its H275Y variant.
Discussion: In addition, in vitro assessment of the oseltamivir H275Y NA mutation seems to reduce viral fitness by means of viral replication when compared with the Wt F virus at the initial stages of infection, as previously described.
Discussion: In general, OR pdm09 viral strains with the H275Y NA mutation are less virulent than Wt viruses.
Discussion: Interestingly, it has been shown that viruses from both the Newcastle and Sapporo clusters contained permissive NA mutations
Successful treatment with baloxavir marboxil of a patient with peramivir-resistant influenza A/H3N2 with a dual E119D/R292K substitution after allogeneic hematopoietic cell transplantation: a case report.
Discussion: In the global surveillance, the frequency of viruses with reduced susceptibility to one or more NA inhibitors has remained low (2012-13: 0.6%; 2013-14: 1.9%; 2014-15: 0.5%; 2015-16: 0.8%; 2016-17: 0.2%), while oseltamivir and peramivir cross-resistant A/H1N1pdm09 viruses with an NA H275Y substitution were the most frequently observed.
Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9.
Abstract: Using the developed method, we successfully identified SARS-CoV-2, pH1N1, and pH1N1/H275Y viruses by the naked eye.
Method: SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020) and pH1N1/H275Y mutant virus (H275Y mutation; A/Korea2785/2009 pdm: NCCP 42017) were provided by the National Culture Collection for Pathogens (NCCP), which is operated by the Korea National Institute of Health.
Method: pH1N1 and pH1N1/H275Y virus titers were determined using a one-step real-time PCR kit (Promega, Madison, WI) in accordance with the manufacturer's instructions.
Result: As shown in Figure 5d, upper well surfaces were coated with dCas9/gRNA (H1) and lower with dCas9/gRNA (H275Y N1).
Result: By adding a mismatched gRNA sequence, the H275Y SNP could be distinguished using th
Characterization of neuraminidase inhibitor-resistant influenza virus isolates from immunocompromised patients in the Republic of Korea.
Discussion: Although NAI-resistant virus has been rarely observed since 2000, the KCDC, through the KINRESS, identified oseltamivir and peramivir-resistant A(H1N1)pdm09 viruses harboring the H275Y NA variation in patients with acute lymphoblastic leukemia and relapsed lymphoma hospitalized in the same general hospital, with both exhibiting prolonged virus excretion.
Discussion: The NAI-resistant A(H1N1) virus harboring
Discussion: demonstrated that A(H1N1)pdm09 virus-infected immunocompromised ferrets exhibited prolonged virus replication despite antiviral therapy, along with the H275Y substitution observed in the virus population from day 8 onwards only in ferrets that received oseltamivir.
Development of A4 antibody for detection of neuraminidase I223R/H275Y-associated antiviral multidrug-resistant influenza virus.
Introduction: According to the experimental and simulation results, the binding affinity of A4 for oseltamivir- and zanamivir-resistant mutant I223R/H275Y NA is approximately 600 times stronger than its binding affinity for oseltamivir- and zanamivir-susceptible wild-type (wt) NA.
Introduction: Here we develop a monoclonal antibody, A4, specific to I223R/H275Y NA.
Introduction: In animal models and in vitro studies, I223R/H275Y double-mutant virus has shown high levels of resistance to oseltamivir and zanamivir, and the combination of I223R with H275Y does not compromise the replication c
Effect of influenza H1N1 neuraminidase V116A and I117V mutations on NA activity and sensitivity to NA inhibitors.
Abstract: The efficiencies of NAs with E119A, H275Y, and N295S mutations to catalyze all substrates were ~19.4% of the CA/04 NA.
Abstract: We compared the impact of V116A and I117V on the functional properties of NA and compared these mutations with that of previously reported NAI-resistant mutations, E119A, H275Y, and N295S.
IV mutation literature information.
PMID: 
2020
Proc Natl Acad Sci U S A
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