Abstract: Here, we identified a new helper mutation, Y276F, that can rescue the functional defects of H275Y and contribute to the evolution of drug resistance in IAV.
Abstract: In this study, we measured the relative fitness, NA activity, and surface expression, as well as sensitivity to oseltamivir, for several oseltamivir resistance mutations, including H275Y in the wild-type and Y276F genetic background.
Abstract: Our results demonstrate that Y276F selectively rescues the fitness defect of H275Y by restoring its NA surface expression and enzymatic activity, elucidating the local compensatory structural impacts of Y276F on the adjacent H2
Design, synthesis, and bioassay of 4-thiazolinone derivatives as influenza neuraminidase inhibitors.
PMID: 33540229
2021
European journal of medicinal chemistry
Abstract: In addition, D41 showed low toxicity and greater potency than reference compounds Oseltamivir and Amantadine against N1-H275Y variant in cellular assays.
Inhibitory Activity of Honeysuckle Extracts against Influenza A Virus In Vitro and In Vivo.
Abstract: The acids-flavonoids mixture had the strongest inhibitory effects in vitro with EC50 values of 3.8, 4.1, and > 20 mug/mL against H1N1, H3N2 and H1N1-H275Y, respectively, showing competitive antiviral activity with oseltamivir and ribavirin.
Abstract: The cytopathic effect reduction assay showed that all the four extracts inhibited the replication of influenza viruses H1N1, H3N2 and the oseltamivir-resistant mutant strain H1N1-H275Y.
Investigation of genetic variation: Neuraminidase gene of influenza A virus H1N1/pdm09, Shiraz, Iran (2015-2016).
Abstract: Based on sequencing results, 2 of the 44 sequenced isolates exhibited H275Y substitution, which presented oseltamivir resistance.
Secondary substitutions in the hemagglutinin and neuraminidase genes associated with neuraminidase inhibitor resistance are rare in the Influenza Resistance Information Study (IRIS).
Abstract: All cultured viruses with the known resistance substitutions H275Y or R292K showed reduced susceptibility to oseltamivir in the NA-star assay.
Abstract: Known resistance substitutions were detected by mutation specific RT-PCR in viruses of 57 of 1803 (3.2%) oseltamivir-treated individuals, including 39 individuals infected with A/H1N1pdm09 [H275Y] virus and 18 with A/H3N2 [R292K] virus.
Abstract: Only in two A/H1N1pdm09 [H275Y] viruses, the potentially compensatory secondary substitutions HA-D52N and NA-R152K were detected.
Nanopore metagenomic sequencing of influenza virus directly from respiratory samples: diagnosis, drug resistance and nosocomial transmission, United Kingdom, 2018/19 influenza season.
Abstract: Furthermore, it was immobilized on Au nanoplate and nanoparticles, enabling surface-enhanced Raman scattering (SERS)-based detection of the H275Y mutant pH1N1.
Abstract: Here we report a novel 6E3 monoclonal antibody capable of recognizing and binding to the H275Y neuraminidase (NA) mutation, which has been associated with reduced susceptibility of influenza viruses to NA inhibitors.
Abstract: Molecular modeling studies also suggest the high-affinity binding of this antibody to pH1N1 H275Y NA.
Abstract: The 6E3 antibody had a KD of 72.74 muM for wild-type NA and 32.76 pM for H275Y NA, suggesting that
Genetic and antigenic characterization of influenza A/H5N1 viruses isolated from patients in Indonesia, 2008-2015.
Result: However, none of the newly isolated viruses encoded known NAI resistance mutations (i.e., V116A, I117V, E119V, G136K, V149A, R156K, D198N, S246N, H275Y, R293K, N295S (N1 numbering)).
Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018-2019 winter season.
PMID: 32459617
2020
Journal of medical microbiology
Method: Screening of A(H1N1)pdm09 viruses for the presence of point mutations encoding NA H275Y amino acid substitution, known to confer oseltamivir resistance, was carried out using a real-time RT-PCR assay that allowed discrimination of a single nucleotide difference between oseltamivir sensitive and resistant viruses.
Result: Testing was performed on 280 detected A(H1N1)pdm09 viruses by real-time RT-PCR with respect to the NA H275Y oseltamivir resistance substitution at the NRL in Bulgaria; NA and PA sequences of all study A(H1N1)pdm09 and A(H3N2) viruses were screened for known markers of reduced susceptibility to NA inhibitors and baloxavir marboxil and phenotypic testing (by the MUNANA method) was performed on 13 influenza A(H1N1)pd
Baloxavir for the treatment of Influenza in allogeneic hematopoietic stem cell transplant recipients previously treated with oseltamivir.
IV mutation literature information.
PMID: 
2022
Journal of virology
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