Result: With the two pairs of ferret antisera elicited by the rgSH2WT/rgSH2-G218E and rgGD17/rgGD-G218E, the two-way hemagglutination inhibition (HI) assay results determined that the HI titers of each pair were
Result: which indicated a more efficient SA cleavage by the rgSH2-G218E than by the rgSH2WT, although the expression of NA on both rgSH2WT and rgSH2-G218E was comparable, as determined by SDS-PAGE.
Result: which is a critical component of RBS, the G218E substitution is likely relevant to alteration in virus binding to host cells.
Figure: (C to E) BLI equilibrium binding to the immobilized 3'SLN or 6'SLN by NL12WT and NL12ad (C), rgSH2WT and rgSH2-G218E (D), and rgGD17 and rgGD-G218E (E).
Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds.
Abstract: After sequential passage in mice, mouse-adapted viruses bearing mutations PB2-E627K and HA-G218E were generated.
Manipulation of neuraminidase packaging signals and hemagglutinin residues improves the growth of A/Anhui/1/2013 (H7N9) influenza vaccine virus yield in eggs.
Abstract: The HA of the passaged CVV, did, however, exhibit egg-adaptive mutations and one of them (HA-G218E) improved CVV growth in eggs without significantly changing antigenicity.
Abstract: The HA-G218E substitution and a chimeric NA, thus, combine to provide an Anhui/1 CVV with properties more favorable for vaccine manufacture.
Enhanced pathogenicity and neurotropism of mouse-adapted H10N7 influenza virus are mediated by novel PB2 and NA mutations.
PMID: 28597818
2017
The Journal of general virology
Abstract: Sequencing showed the absence of the widely recognized mammalian adaptation markers of E627K and D701N in PB2 in the mouse-adapted strain; instead, five amino acid mutations were identified: E158G and M631L in PB2; G218E in haemagglutinin (H3 numbering); and K110E and S453I in neuraminidase (NA).
Adaptive amino acid substitutions enhance the virulence of a reassortant H7N1 avian influenza virus isolated from wild waterfowl in mice.
Abstract: Analysis of the variant virus genomes revealed amino acid changes in the PB2 (E627K), HA (H3 numbering; E114K, G205E, and G218E), and NA (S350N) proteins.
Resistance to neuraminidase inhibitors conferred by an R292K mutation in a human influenza virus H7N9 isolate can be masked by a mixed R/K viral population.
Abstract: Non-conservative mutations in the hemagglutinin (Gly218Glu) and non-structural 1 (Asp125Gly) proteins were identified in P10 virus which exhibited high virulence.
IV mutation literature information.
PMID: 
2019
Journal of virology
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