Result: The calculated apparent dissociation constants of wild-type and Phe95 Tyr HA are 5.8 x 10-3 M and 3.5 x 10-6 M for the 3'SLN-LN, 2.3 x 10-4 M and 1.6 x 10-11 M for the 6'SLN-LN, respectively (Table 1).
Result: The ectodomains of the recombinant wild-type influenza B/Yamagata/73 HA and its mutants (single mutants: Phe95 Tyr, Asn194 Asp and double mutant Phe95 Tyr/Asn194 Asp) were expressed in mammalian cell CV-1 using vaccinia virus system and purified to high purity.
Result: The instability of Asn194 of recombinant wild-type B/Le
Result: We next tested whether the enhanced receptor-binding affinity of the Phe95 Tyr and Phe95 Tyr/Asn194 Asp mutants allows influenza B/Yamagata/73 HA to bind host cells significantly better.
IV mutation literature information.
PMID: 
2014
Virology
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