Abstract: Moreover, 44 strains had A558V mutations in the PB2 protein and four had E627V mutations, along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.
Double mutations in the H9N2 avian influenza virus PB2 gene act cooperatively to increase viral host adaptation and replication for human infections.
PMID: 34061017
2021
The Journal of general virology
Abstract: K526R, E627V or E627K), indicating a host adaptation advantage for these double mutations.
Dynamic PB2-E627K substitution of influenza H7N9 virus indicates the in vivo genetic tuning and rapid host adaptation.
Discussion: E627V is reportedly associated with H5N1 adaptation in MDCK cells and can also enhance H9N2 polymerase activity in human cells and mouse virulence.
Discussion: We found a linked and simultaneous adaptation of 701E with 627V in the PB2 of H7N9 viruses from seven patients in Shenzhen, possibly indicating that D701E coemerges on the same alleles carrying E627V in the virus quas
Discussion: showed that PB2-E627V/E543D/A655V/K526R mutations act cooperatively to produce the high polymerase activity of PB2 and efficient replication of the H9N2 virus in human cells and to increase replication and pathogenicity in mice in vivo.
PA Mutations Inherited during Viral Evolution Act Cooperatively To Increase Replication of Contemporary H5N1 Influenza Virus with an Expanded Host Range.
Introduction: In addition, we recently reported that the H9N2 virus in the Middle East (G1-A/B subclade), centered in Egypt, commonly carries the PB2-E627V, -E543D, -A655V, and -K526R mutations, which all contributed to more fit replication in human cells and mice.
Introduction: Other mammal adaptation mutations include PB2-D701N, -K526R, -Q591K, -E192K, -K702R, and -E627V.
Discussion: The PB2 genes from clade 2.2.1.2 and H9N2 G1-A/B subclade viruses in Egypt have commonly acquired PB2-
In silico thermodynamic stability of mammalian adaptation and virulence determinants in polymerase complex proteins of H9N2 virus.
Discussion: The PB2 protein displayed valine at position 627 (E627V) is typical of G1 viruses lineage, group A.
Discussion: The substitutions (K318R, I504V, 647I, E627V, and A661 T) Also, were detected suggesting increased polymerase activity and virus replication concluding that the PB2 subunit of the LPAI-H9N2 viruses.
Discussion: This mutation (E627V) suggested increasing H5N1 virus replication in mammalian cells and virulence in mice compared with PB2-627E.the three mutations (L13P, V14A, 317I, and 473V) in PB1 protein affect the virus replication in mammal
IV mutation literature information.
PMID: 
2021
Virologica Sinica
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