literature

Mutations in polymerase genes enhanced the virulence of 2009 pandemic H1N1 influenza virus in mice.

PMID: 22438920 2012 PloS one

Introduction: Synergistic effect of mutations in PA (F35L) and HA (D222G and K163E) was recently reported in a mouse-adaptive pH1N1 virus.

Introduction: The D222G substitution in pH1N1 HA, occurring either naturally or as a mouse-adaptation, was associated with severe or fatal disease in mice by altering cell tropism.

Discussion: A synergistic effect on polymerase activity rendered by dual PA-36T and PB2-357N substitutions was observed (data not shown) and the mutant containing PA-A36T and PB2-H357N accompanied with

PMID: 22102733 2012 The Journal of infectious diseases

Abstract: Using reverse genetics, 3 synergistically acting mutations were defined as pathogenicity determinants, comprising 2 mutations in the hemagglutinin (HA[D222G] and HA[K163E]), whereby the HA(D222G) mutation was shown to determine receptor binding specificity and the polymerase acidic (PA) protein F35L mutation increasing polymerase activity.

Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010.

PMID: 22363521 2012 PloS one

Abstract: In May 2009, three specimens from mild cases showed D222G and/or Q223R substitutions in a minor subpopulation of viruses infecting these individuals.

Abstract: METHODS/RESULTS: To elucidate the existence and transmissibility of alpha2,3 sialic acid-specific viruses in H1N1pdm, amino acid substitutions within viral hemagglutinin molecules were investigated, especially D187E, D222G, and Q223R, which are related to a shift from human to avian receptor specificity.

Introduction: In addition to the D222G substitution, we focused on two other substitutions {aspartic acid187glutamic acid (D187E) and glutamine223argine (Q223R)}, kn

Neuraminidase H275Y and hemagglutinin D222G mutations in a fatal case of 2009 pandemic influenza A (H1N1) virus infection.

PMID: 22243670 2012 Influenza and other respiratory viruses

Abstract: Further investigations are needed to determine the significance of infection with strains possessing NA-H275Y and HA-D222G.

Abstract: Strains possessing the hemagglutinin (HA) D222G mutation have been detected in small numbers of fatal 2009 H1N1 cases.

Abstract: We report the first clinical description of 2009 H1N1 virus infection with both NA-H275Y and HA-D222G mutations detected by pyrosequencing of bronchioalveolar lavage fluid obtained on symptom day 19.

[Cuban strategy for the molecular characterization of the pandemic influenza A virus (H1N1)].

PMID: 23437533 2011 Revista cubana de medicina tropical

Abstract: RESULTS: The third strategy provided the most comprehensive results such as differential diagnosis, the surveillance of the D222G/E mutation in hemagglutinin and Tamiflu-resistant H275Y viral variants.

[Pathogenic aspects of influenza during the epidemics caused by A/H1N1v virus in 2009-2010 according autopsy data].

PMID: 22379895 2011 Arkhiv patologii

Abstract: In the majority of investigated strains was proved the amino acid mutation with replacement D222G.

[Genetic variability of isolates of pandemic influenza A virus H1N1 isolated in Russia in 2009].

PMID: 22312897 2011 Molekuliarnaia genetika, mikrobiologiia i virusologiia

Abstract: The hemagglutinin gene mutation D222G was found in 4 isolates from autopsy material.

Minor changes in the hemagglutinin of influenza A(H1N1)2009 virus alter its antigenic properties.

PMID: 22022458 2011 PloS one

Introduction: Other mutations, such as D127E, S183P and D222G have been shown to be associated with a more virulent phenotype in humans or mice.

Effect of D222G mutation in the hemagglutinin protein on receptor binding, pathogenesis and transmissibility of the 2009 pandemic H1N1 influenza virus.

PMID: 21966421 2011 PloS one

Resul

Result: Effect of D222G mutation on pathogenesis and respiratory droplet transmission.

Result: Effect of D222G mutation on the receptor binding properties of HA.

Genetic structure of human A/H1N1 and A/H3N2 influenza virus on Corsica Island: phylogenetic analysis and vaccine strain match, 2006-2010.

PMID: 21935413 2011 PloS one

Discussion: The D222G mutation was not observed among A/H1N1pdm isolated on Corsica Island.