literature

IV mutation literature information.

H7N9 Influenza Virus Containing a Polybasic HA Cleavage Site Requires Minimal Host Adaptation to Obtain a Highly Pathogenic Disease Phenotype in Mice.

PMID: 31948040 2020 Viruses

Abstract: In addition to the PBC site, we identified three other mutations that are important for host-adaptation and virulence in mice: HA (A452T), PA (D347G), and PB2 (M483K).

Method: Amino acid mutations in HA (A452T, H7 HA numbering with the signal peptide included), PA (D347G), and PB2 (M483K) were also inserted into each respective pPol vector by PCR using PrimeSTAR Max and in-fusion cloning.

Result: Although introduction of D347G (PA) or

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