Abstract: Among the high-level variations found in ICC, six were located in the E1/E2 genes, and all of them were non-synonymous substitutions (Q142K, M207I, and L262V for E1; D153Y, R302T, and T357A for E2).
Result: Because all E2 proteins so far reported have a threonine residue at position 357, we examined E2 T357A instead of A357T.
Result: In C33A cells, R302T was less efficiently expressed than t