Method: The mutations were done as indicated next, to obtained E-G350: L83V; E-C188/G350: E29Q and L83V; E-A176/G350: D25N and L83V; AAa: Q14H, H78Y, and L83V; for AAc: Q14H, I27R, H78Y, and L83V.
Result: E6 mutations Q14H, D25N, I27R, E29Q and H78Y are found in a non-domain region adjacent to the zinc finger domain 1.
Figure: (C) Visualization of amino acid changes:
Genetic variability of the HPV16 early genes and LCR. Present and future perspectives.
PMID: 34847982
2021
Expert reviews in molecular medicine
Abstract: Nowadays, specific mutations of HPV16 DNA have been associated with an increased risk of high-grade squamous intraepithelial lesions and invasive cervical cancer (ICC) development, including E6: Q14H, H78Y, L83V, Epsilon7: N29S, S63F, E2: H35Q, P219S, T310K, E5: I65V, whereas highly conserved regions of viral DNA have been extensively characterised.
Phylogenetic analysis of Human papillomavirus 16 variants isolated from Indian Breast cancer patients showed difference in genetic diversity with that of cervical cancer isolates.
Abstract: Whereas, 145 G>T (Q14H) and 335 C>T (H78Y) variants of E6 showed association with either early invasiveness of BC and/or poor outcome of the patients.
Association of human papillomavirus 16 E6 variants with cervical carcinoma and precursor lesions in women from Southern Mexico.
Introduction: Other polymorphisms including A131G, Table: Q14H
Discussion: According to the HPV variants found in this study, the amino acid substitutions circulating in Southern Mexico are: Q3E, R10G, Q14H, D25N, I27L, I27R I27T, L28V, E29K, E29Q, E29G, I52L, K68T, F69L, H78Y, L83V (the most frequent) and E113D.
Genetic variability in E6 and E7 oncogenes of human papillomavirus Type 16 from Congolese cervical cancer isolates.
Abstract: To elucidate the functional effects of this polymorphism, we followed keratinocytes transduced with E-T350G E6 for over 60 passages and compared them to keratinocytes transduced, in parallel, with prototype or Asian-American (Q14H/L83V/H78Y) E6.
Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women.
Result: The other non-synonymous variants were R10I (G132T), R10G (A131G), D64E (T295G), Q14H (G145
Discussion: Additionally, E6 variants R10G/L83V and Q14H/H78Y/L83V which are frequently detected in our study, were more prone to undergo cell-detachment-induced apoptosis than E6 prototype in a model of human normal immortalized keratinocytes (NIKS) and has a greater in vitro ability to suppress keratinocyte differentiation responses and to induce p53 degradation than the prototype.
Human papillomavirus (HPV) 16 E6 variants in tonsillar cancer in comparison to those in cervical cancer in Stockholm, Sweden.
Introduction: In a previous study we investigated the functional characteristics of L83V-related
Discussion: High activity of the Asian American variant Q14H/H78Y/L83V in apoptosis induction was suggested to be related to its high persistence and increased risk of progression to cancer (reported to be 20-fold higher than that of prototype E6).
Discussion: In an earlier investigation NIKS transduced with the E6 variants Q14H/H78Y/L83V and R10G/L83V demonstrated significantly higher levels of late apoptotic cells when suspended in methylcellulose than L83V.
Gene expression profiles are altered in human papillomavirus-16 E6 D25E-expressing cell lines.
HPV mutation literature information.
PMID: 
2022
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