Result: Sequence analysis of the E6 gene in 25 HPV 16 positive ICC cases showed the presence of the T350G mutation in the great majority (18/25, 72%), in 4 cases also accompanied by other mutations; among the mutations identified, A257G, C335T, T350G, A442C lead to amino acid changes (I52V, H78Y, L83V, E113D, respectively), while C256T, T286A, A289G are silent not leading to amino acid changes.

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