literature

HIV mutation literature information.

Next generation sequencing improves detection of drug resistance mutations in infants after PMTCT failure.

PMID: 25542470 2015 Journal of clinical virology

Abstract: RESULTS: Of 15 infants, tested at a median age of 3.4 months after birth, 2 (13%) had non-nucleoside reverse transcriptase inhibitor (NNRTI) DRMs (K103N and Y181C) by bulk sequencing, whereas PGM detected 4 (26%) and MiSeq 5 (30%).

Introduction: A recent study found good correlation of Roche 454 sequencing for K103N and Y181C, when screening PMTCT exposed children (less than 2 years of age) prior to cART initiation.

Result: For the infant with K103N, detected by bulk sequencing, both Ion PGM and MiSeq additionally detected V106A/M and Y181C DRMs.

Fused heterocycles bearing bridgehead nitrogen as potent HIV-1 NNRTIs. Part 3: optimization of [1,2,4]triazolo[1,5-a]pyrimidine core via structure-based and physicochemical property-driven approaches.

PMID: 25626145 2015 European journal of medicinal chemistry

Abstract: Additionally, 7d demonstrated weak activity against the double mutant HIV-1 strain (K103N + Y181C), and was more efficient than NVP in a RT inhibition assay.

Prevention of mother-to-child HIV-1 transmission in Burkina Faso: evaluation of vertical transmission by PCR, molecular characterization of subtypes and determination of antiretroviral drugs resistance.

PMID: 25630709 2015 Global health action

Abstract: Genetic mutations were also isolated that induce resistance to ARV such as M184V, Y115F, K103N, Y181C, V179E, and G190A.

Introduction: have already emphasized the context by which PMTCT led to resistance mutations induced by nevirapine (NVP; V8IV, K103N, V179E, and Y

Table: Y181C

A novel mutation, D404N, in the connection subdomain of reverse transcriptase of HIV-1 CRF08_BC subtype confers cross-resistance to NNRTIs.

PMID: 25637519 2015 The Journal of antimicrobial chemotherapy

Abstract: Double mutations Y181C/D404N and Y181C/H221Y significantly reduced susceptibility to NNRTIs.

Abstract: The most pronounced resistance to NNRTIs was observed with the triple mutation Y181C/D404N/H221Y.

High prevalence of the K65R mutation in HIV-1 subtype C infected patients failing tenofovir-based first-line regimens in South Africa.

PMID: 25659108 2015 PloS one

Result: In contrast the Y181C mutation was found in 47.1% (n = 32) and 6.5% (n = 6) of the NVP and EFV group respectively (aRR 0.16 95% CI 0.08-0.35.

Table: Y181C

Effects of HIV-1 reverse transcriptase connection subdomain mutations on polypurine tract removal and initiation of (+)-strand DNA synthesis.

PMID: 25662223 2015 Nucleic acids research

Introduction: K103N or Y181C) (reviewed in).

Structure-based evaluation of non-nucleoside inhibitors with improved potency and solubility that target HIV reverse transcriptase variants.

PMID: 25700160 2015 Journal of medicinal chemistry

Method: Final clones for the RT (Y181C) and RT (K103N/Y181C) were verified by DNA sequencing.

Method: For the RT (K103N/Y181C):1 complexes, crystals were first obtained using cocrystallization then dehydrated using similar methods described previously.

Method: For the RT (Y181C):1, RT (Y181C):2, and RT (K103N/Y181C):2 complexes, crystal soaking techniques were used to obtain the C2 crystal forms.

Predicted residual activity of rilpivirine in HIV-1 infected patients failing therapy including NNRTIs efavirenz or nevirapine.

PMID: 25704446 2015 Clinical microbiology and infection

Abstract: Most prevalent RPV-RAMs after nevirapine experience were Y181C and H221Y, whereas L100I+K103N, Y188L and K101E occurred most in efavirenz-experienced patients.

Characterization of HIV drug resistance mutations among patients failing first-line antiretroviral therapy from a tertiary referral center in Lusaka, Zambia.

PMID: 25754408 2015 Journal of medical virology

Abstract: Analysis of reverse transcriptase revealed M184V (88%), K103N/S (32%), and Y181C/I/V (41%) DRMs, with the latter conferring reduced susceptibility to the salvage therapy candidates etravirine and rilpivirine.

Result: Of note, there was a high prevalence of Y181C/I/V (n = 28, 41% cumulatively) that confers reduced susceptibility to both rilpivirine (RPV) and etravirine (ETR), and Y188L (n = 5, 7%) which leads to reduced susceptibility to RPV.

Result: The most prevalent NNRTI mutations were Y181C/I/V (n = 24, 35%), (n = 3, 4%), and (n = 1 (1%), respectively, K103N/S (n = 21 (32%) and n = 1 [1%]),

PMID: 25788547 2015 Journal of clinical microbiology

Abstract: ARMS-PCR's limits of detection for mutations M184V, T215Y/F, K103N, and Y181C were <75 copies/ml, 143 copies/ml, 143 copies/ml, and 836 copies/ml, respectively.

Abstract: The ARMS-PCR assay was able to detect M184V, T215Y/F, K103N, and Y181C mutations with sensitivities of 96.8%, 85.7%, 91.3%, and 70%, respectively, and specificities of 90.6%, 95%, 100%, 96.9%, respectively, compared with data on sequencing.

Browser Board

Co-occurred Entities

Filtrator