Allosteric modulation of the HIV-1 gp120-gp41 association site by adjacent gp120 variable region 1 (V1) N-glycans linked to neutralization sensitivity.
Discussion: The generation of functional Env complexes with stable gp120-gp41 association following the combination of V1 glycan mutations with various L593V, W596L or K601D
Discussion: The impacts of the V1 glycan changes on the gp120-gp41 association site were probed further by combining T138N, L494I and DeltaN139INN with various DSR mutations: L593V and K601D, which disrupt gp120-gp41 association and fusion function, and W596L which inhibits fusion.
Forced virus evolution reveals functional crosstalk between the disulfide bonded region and membrane proximal ectodomain region of HIV-1 gp41.
Result: The W596L mutation was retained in 70/71 env clones obtained from the WLKD-1 and WLKD-2 cultures indicating a strong selection pressure to maintain Leu at 596.
Figure: Lysates of metabolically lab
Discussion: Interestingly, the addition of D674E to W596L/K601H only marginally improved gp120-association for cell-free virions whereas single-cycle infectivity was improved some 20-fold.
Discussion: It is interesting that the boosts to infectivity associated with the addition of D674E to W596L/K601H did not correlate with enhanced cell-cell fusion activity, where the cell-surface expressed glycoproteins function independently of other virion components.
Role for the disulfide-bonded region of human immunodeficiency virus type 1 gp41 in receptor-triggered activation of membrane fusion function.
PMID: 20230797
2010
Biochemical and biophysical research communications
Abstract: The introduction of W596L and W610F mutations to the DSR of HIV-1(QH1549.13) blocked viral entry and hemifusion without affecting gp120-gp41 association.
HIV mutation literature information.
PMID: 
2013
PLoS pathogens
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