Result: Error rates of 3.3 x 10-5 were estimated for the O_K65R/V75I RT under those conditions (Table 4).
Result: In previous studies, we found that the mutant O_K65R/V75I RT was >15-fold more accurate than the HIV-1BH10 RT in forward mutation assays measuring fidelity of DNA-dependent DNA synthesis.
Result: Interestingly, the analysis of mutational spectra revealed that major hotspots obtained with O_K65R/V75I RT while reverse transcribing an RNA template synthesized at pH 7.9/6 mM Mg2.
Result: Interestingly, the spectrum of the O_K65R/V75I RT sh
Distinct Pattern of Thymidine Analogue Mutations with K65R in Patients Failing Tenofovir-Based Antiretroviral Therapy.
PMID: 29084434
2018
AIDS research and human retroviruses
Introduction: However, in the 31 patients with no K65R present at S2, 6 had intermediate or high-level resistance to AZT: 4 were caused by TAM-2 DRMs, 1 by T215Y, and 1 by Q151M-complex mutations Q151M, A62V, V75I, F77L, F116Y.
Characterization of minority HIV-1 drug resistant variants in the United Kingdom following the verification of a deep sequencing-based HIV-1 genotyping and tropism assay.
Result: Most of the minority mutations in viruses from both groups of naive patients were observed in the RT, e.g., M41L, E44D, A62V, K65R, D67N, D67G, V75I, L100I, K103N, K103R, V188I, M184I, L210W, K219Q, Y318F, etc., although a number of minority mutations associated with resistance to PI (L10F, V11I, M46I/
Virological response, HIV-1 drug resistance mutations and genetic diversity among patients on first-line antiretroviral therapy in N'Djamena, Chad: findings from a cross-sectional study.
Fidelity of classwide-resistant HIV-2 reverse transcriptase and differential contribution of K65R to the accuracy of HIV-1 and HIV-2 reverse transcriptases.
Result: Previous studies with prototypic HIV-1 RTs had shown that K65R produced large increases in the fidelity of DNA synthesis in the absence and in the presence of the NRTI-associated resistance mutation V75I.
Discussion: Furthermore, studies with the group O enzyme also showed the increased nucleotide selectivity of the double-mutant K65R/V75I in comparison with the RT bearing the single amino acid substitution V75I.
Mutational Correlates of Virological Failure in Individuals Receiving a WHO-Recommended Tenofovir-Containing First-Line Regimen: An International Collaboration.
Result: Five NRTI-associated TRAMs occurred significantly more commonly in the thymidine analog group including V75I, T165L, M184V, D218E and L228H.
Result: Of the 83 TRAMs, 16 (21%; 16/83) were established NRTI-associated resistance mutations including A62V, K65R/N, T69deletion, K70E/Q/T/N, L74V/I, V75M/I/L, Y115F, and M184V/I and 40 (48%; 40/83) were established NNRTI-associated resistance mutations.
Structural Insights into HIV Reverse Transcriptase Mutations Q151M and Q151M Complex That Confer Multinucleoside Drug Resistance.
PMID: 28396546
2017
Antimicrobial agents and chemotherapy
Abstract: Q151M and four associated mutations, A62V, V75I, F77L, and F116Y, were detected in patients failing therapies with dideoxynucleosides (didanosine [ddI], zalcitabine [ddC]) and/or zidovudine (AZT).
Impact of HIV-1 Integrase L74F and V75I Mutations in a Clinical Isolate on Resistance to Second-Generation Integrase Strand Transfer Inhibitors.
PMID: 28533248
2017
Antimicrobial agents and chemotherapy
Abstract: A novel HIV-1 integrase mutation pattern, L74F V75I, which conferred resistance to first-generation integrase strand transfer inhibitors (INSTIs), was identified in a clinical case with virological failure under a raltegravir-based regimen.
Abstract: Addition of L74F V75I to N155H or G140S Q148H increased resistance levels to the second-generation INSTIs dolutegravir (>385- and 100-fold, respectively) and cabotegravir (153- and 197-fold, respectively).
Use of Proviral DNA to Investigate Virus Resistance Mutations in HIV-infected Zimbabweans.
ViMRT
HIV mutation literature information.
PMID: 
2018
Scientific reports
