Multimethod Longitudinal HIV Drug Resistance Analysis in Antiretroviral-Therapy-Naive Patients.
PMID: 28659324
2017
Journal of clinical microbiology
Abstract: ART-naive HIV-1-infected patients from Cameroon were subjected to a multimethod HIVDR analysis using amplification-refractory mutation system (ARMS)-PCR, Sanger sequencing, and longitudinal next-generation sequencing (NGS) to determine their profiles for the mutations K103N, Y181C, K65R, M184V, and T215F/Y.
HIV-1 viraemia and drug resistance amongst female sex workers in Soweto, South Africa: A cross sectional study.
Deep Sequencing of HIV-1 RNA and DNA in Newly Diagnosed Patients with Baseline Drug Resistance Showed No Indications for Hidden Resistance and Is Biased by Strong Interference of Hypermutation.
PMID: 27076656
2016
Journal of clinical microbiology
Abstract: Despite focused selection of patients with T215 revertants or singleton mutations, deep sequencing failed to identify the resistant T215Y/F or M184V or any other resistance mutation, indicating that in most of these cases there is no hidden resistance and that the virus detected at diagnosis by population sequencing is the original infecting variant.
HIV-1 Transmitted Drug Resistance Mutations in Newly Diagnosed Antiretroviral-Naive Patients in Turkey.
PMID: 26414663
2016
AIDS research and human retroviruses
Abstract: However, TAMs were divided into three categories and M41L, L210W, and T215Y mutations were found for TAM1 in 97 (7.4%) patients, D67N, K70R, K219E/Q/N/R, T215F, and T215C/D/S mutations were detected for TAM2 in 52 (3.9%) patients, and M41L + K219N and M41L + T215C/D/S mutations were detected for the TAM1 + TAM2 profile in 22 (1.7%) patients, respectively.
Biochemical characterization of a multi-drug resistant HIV-1 subtype AG reverse transcriptase: antagonism of AZT discrimination and excision pathways and sensitivity to RNase H inhibitors.
Conclusion: Analyses with subtype B RT indicated that K65R and the TAM T215F/Y are not compatible in the absence of the Q151M complex and were rarely found if Q151M or Q151M and at least two additional mutations of the complex were present.
Conclusion: Thus, a strong functional antagonism of K65R and T215F/Y in the presence of two or more additional TAMs may exist.
Conclusion: We show here, that s
Conclusion: the HIV Drug Resistance Database (http://hivdb.stanford.edu/), which give high AZT resistance mutation scores for Q151M occurring in coexistence with the TAMs T215F and M41L.
Transmission dynamics of HIV-1 subtype B in the Basque Country, Spain.
PMID: 26921800
2016
Infection, genetics and evolution
Abstract: The most prevalent mutations for each inhibitor class were PI L90M, NRTI T215D/Y/F, and NNRTI K103N, which were also among the most prevalent resistant variants in the whole dataset.
Resolution of Specific Nucleotide Mismatches by Wild-Type and AZT-Resistant Reverse Transcriptases during HIV-1 Replication.
Method: To construct an AZTR RT-containing version of pCMVDeltaR8.2, the following amino acid substitutions that confer resistance to AZT: D67N, K70R, T215F, and K219Q were introduced into an HIV reverse transcriptase (RT) gene fragment using overlap extension PCR, sequenced, and used to replace wild-type sequences between AgeI and BclI in pCMVDeltaR8.2 to produce pCBN103-18, which contains an AZT-resistant (AZTR) RT gene.
Rapid and Simultaneous Detection of Major Drug Resistance Mutations in Reverse Transcriptase Gene for HIV-1 CRF01_AE, CRF07_BC and Subtype B in China Using Sequenom MassARRAY(R) System.
Abstract: In terms of loci, the detection rate of the alleles was greater than 97% for M41L, K65R, M184V and G190A, 91.2% for K101E/Q/P, 91.2% for T215F/Y, 89.9% for K103N/S and 80.5% for L210W.
Introduction: In this study, we established a multiplex assay for detecting the drug resistance mutations at 8 loci (M41L, K65R, K101E/Q/P, K103N/S, M184V, G190A, L210W an
Table: T215F/Y
HIV-1 Epidemiology, Genetic Diversity, and Primary Drug Resistance in the Tyumen Oblast, Russia.
Discussion: Among them, the mutations G190S, K103N, M184V, Y181C, K101E, M41L, and T215F/Y, influencing the HIV resistance to NRTIs and NNRTIs, are observed at a high rate.
Surveillance of HIV Transmitted Drug Resistance in Latin America and the Caribbean: A Systematic Review and Meta-Analysis.
Result: This decreasing trend was associated with reductions in frequency of several DR mutations including D67N, K70R, M184V, L210W, T215F, T215Y, and K219E (p<0.05; Fig 5).
Discussion: 3.1%, p<0.0001), with a significant reduction in the frequency of M184V and of most thymidine analogue mutations (TAM), including D67N, K70R, L210W, T215F, T215Y, and K219E.
HIV mutation literature information.
PMID: 
2017
Journal of clinical microbiology
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