Figure: No fitness costs of the early CD8+ T cell escape mutation R355K in Env.
Discussion: One CD8+ T cell escape mutation (
Discussion: The lack of measurable fitness cost for the R355K mutation was in consistent with previous observations that R355K escaped rapidly after transmission and was in a high entropy epitope.
Discussion: The lack of measurable fitness cost of the R355K mutation might facilitate its rapid escape kinetics in vivo and the quick predominance in the viral population throughout the follow-up period.
Discussion: This was in agreement with our in vivo observation that the R355K mutation persisted without reversion after the T cell responses targeting the Env352-360 epitope vanished.
Impact of immune escape mutations on HIV-1 fitness in the context of the cognate transmitted/founder genome.
Method: The tat/env fragment was amplified by using the PCR primers R-lower: 5' Acry-GGAAGCACCCAGGAAGTCAGC-3' (nt 5862-5882) and R-upper: 5'-GTATCCTCTGATGGGAGGGGCATA-3' (nt 7527-7550), and the amplicons were annealed with the sequencing primer Rev7: 5'-ATGCTACTTACTGCTTTGGTAGAGGCGCTTGATTA-3' (nt 6022-6056) to detect the I64T mutation or the sequencing primer Rev13: 5'-CCTCCTGAGGAATGGTTAAAGACTAT-3' (nt 7299-7324) to detect the R355K mutation.
D
Discussion: We determined the fitness cost of two CTL escape mutations (R355K in Env and T242N in Gag) in the context of other mutations in the cognate viral genomes (TK and NIA, respectively).
HIV mutation literature information.
PMID: 
2014
PloS one
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