Result: Quantitative analysis indicated that VLP release efficiency was inhibited an average of 10-fold compared to P7L (n = 7 using 3 independent constructs of P7L-S40F-Gag; to ensure that this result was due to S40F, the entire Gag gene was sequenced).
Result: Supporting this, examination of the CA[EE75,76AA]- Result: When tested on the triple drop-out media (TDO, Trp, Leu and His), which tests for protein-protein interaction, the positive controls [i.e., full-length Alix paired with P7L-Gag, Tsg101 paired with WT Gag, and T Ag paired with p53 (lanes 1, 7 or 9; 3 and 5, respectively)] interacted, as expected.
Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process.
PMID: 18723511
2008
The Journal of biological chemistry
Abstract: In contrast to ASV Gag, HIV-1 Gag containing an L domain inactivating mutation (P7L) was efficiently rescued by fusion to a component of ESCRT-III (Chmp6) but not ESCRT-II (Eap20).
HIV mutation literature information.
PMID: 
2013
Retrovirology
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