Abstract: Primary INSTIs resistance mutation were absent, however secondary mutations L74IM, T97A were detected in four samples (5.2%).
Introduction: None of the primary amino acid mutations in IN positions 66, 92, 140, 143, 147, 148 and 155 listed in the Stanford resistance algorithm were found in this study, while secondary mutations L74IM and T97A associated with drug resistance to RAL and EVG were observed only in four patients (5.2%) (Additional file 1).
Introduction: The L74I was observed in two strains, a subtype B (KU609297) and a subtype A (KU609282).
Selective resistance profiles emerging in patient-derived clinical isolates with cabotegravir, bictegravir, dolutegravir, and elvitegravir.
Result: Selection of strain E78004 with CAB resulted in high level resistance along a Q148R/E138K/G140GS/L74I pathway (Table 4).
Result: Similarly, 96USSN20 and E78004 viruses developed resistance along a Q148R pathway leading to L74M/E138K/G148R/R263K and L74I/E138K/G140S/Q148R conferring cross-resistance to all INSTIs.
Result: The outgrowth of the L74I/E138K/G140GS,
The High Genetic Barrier of EFdA/MK-8591 Stems from Strong Interactions with the Active Site of Drug-Resistant HIV-1 Reverse Transcriptase.
Introduction: Moreover, we found that nine amino acid substitutions, namely, M41L, D67Delta, T69G, K70R, L74I, V75T, M184V, T215F, and K219Q (in HIV-1EFdAR), were associated with EFdA resistance.
Method: Recombinant HIV-1 clones (HIV-1K65R, HIV-1A114V, HIV-1Y115A, HIV-1F160A, HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/ PMID: 30409215
2018
AIDS research and therapy
Result: As expected, the number and type of mutations with frequencies > 20% (those within Sanger sequencing-based detection level) matched the cART history of the patient, e.g., the virus from patient SG28 had mutations associated with resistance to ABC (L74I 97.6% frequency), 3TC (M184V 99.7%), and EFV (K103N 98.8%, P225H 99.5%) and had been exposed to RTV, DRV, ABC, 3TC, and EFV; while patient SG86 had a virus with resistance to FTC (M184V 98.9%) and RAL (L74M 97.9%, E92Q 86.1% and T97A 99.8%) after being treated over the years with RTV, LPV, DRV, FTC, TDF, and RAL (Additional file 1: Table S1).
Result: Most of the minority mutations in viruses from both groups of naive patients were observed in the
Performance of Celera RUO integrase resistance assay across multiple HIV-1 subtypes.
PMID: 27993614
2017
Journal of virological methods
Abstract: Mutations associated with integrase resistance were observed in seven of the 106 (7%) clinical samples [one sample: Q148K; E138K; G140A; two samples: T97A and four samples: L74I].
Abstract: Of the four samples with L74I, 3 were subtype G.
Result: Of the four samples with L74I, three were subtype G.
Result: The following mutations were observed: Q148K; E138K; G140A in one sample; T97A in two samples; and L74I in four samples.
Discussion: This difference could be a result of subtype specific polymorphism as
Polymorphisms and Mutational Covariation Associated with Death in a Prospective Cohort of HIV/AIDS Patients Receiving Long-Term ART in China.
Abstract: Among them, 7 polymorphisms (L74I, K103N, V106A, Y181C, G190A, T215I and P225H) were known to be drug resistance mutations, 7 polymorphisms (E6D, E35D, S37N, I93L, E169D, T200V and T200E were considered to be potential drug resistance mutations, and 6 polymorphisms (T39A, K43E, S68N, Q197K, T200V and E22
HIV-1 drug-resistant mutations and related risk factors among HIV-1-positive individuals experiencing treatment failure in Hebei Province, China.
Temporal Patterns and Drug Resistance in CSF Viral Escape Among ART-Experienced HIV-1 Infected Adults.
PMID: 28328546
2017
Journal of acquired immune deficiency syndromes (1999)
Result: Two studies reported sequencing the integrase gene in CSF; N155H, L74I, and Q148R mutations were observed as single mutations in the integrase gene, and Y143C was observed in combination with T66I.
Mutational Correlates of Virological Failure in Individuals Receiving a WHO-Recommended Tenofovir-Containing First-Line Regimen: An International Collaboration.
Result: Of the 83 TRAMs, 16 (21%; 16/83) were established NRTI-associated resistance mutations including A62V, K65R/N, T69deletion, K70E/Q/T/N, L74V/I, V75M/I/L, Y115F, and M184V/I and 40 (48%; 40/83) were established NNRTI-associated resistance mutations.
Result: The NRTI-associated TRAMs most commonly correlated with other NRTI-associated TRAMs were K65R (n = 10 pairs), M184V (n = 10 pairs), Y115F (n = 7 pairs), A62V (n =
Human Immunodeficiency Virus-1 Viral Load Is Elevated in Individuals With Reverse-Transcriptase Mutation M184V/I During Virological Failure of First-Line Antiretroviral Therapy and Is Associated With Compensatory Mutation L74I.
PMID: 28383390
2017
The Pediatric infectious disease journal
Method: Association of antiretroviral and L74V/I mutations were assessed by the Fisher's exact test.
Result: All 37 children with L74V/I mutations also had M184V and thus high-level resistance to ABC from this combination of mutations.
Result: Detection of L74V/I was associate
Discussion: L74 mutations alone cause intermediate-level resistance to ABC but when combined with the common mutation M184V as was the case for every child with identified L74V/I in our study, this causes high-level resistance to ABC and likely necessitates a change in the ART regimen to regain virologic suppression.
Discussion: We found that almost all children on ART with VF and L74V/I mutations were on ABC-containing regimens.
HIV mutation literature information.
PMID: 
2018
BMC research notes
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