Human Immunodeficiency Virus-1 Viral Load Is Elevated in Individuals With Reverse-Transcriptase Mutation M184V/I During Virological Failure of First-Line Antiretroviral Therapy and Is Associated With Compensatory Mutation L74I.
PMID: 31774913
2020
The Journal of infectious diseases
5Result: A previous study reported that L74I can restore replication to a virus with the K65R mutation without conferring drug resistance; therefore, we sought to test the hypothesis that L74I could restore replication ""fitness"" to a M184V mutant virus, thereby explaining the higher than expected VLs."
Method: We repeated this process in subgroups of patients defined by several baseline characteristics: presence of K65R mutation, presence of major NNRTI mutations, choice of NRTI, choice of NNRTI, categories of baseline CD4 count (< and >200 cells/mm3), and categories of baseline VL (< and >100 000 copies per mL).
Result: Many of these mutations have previously been associated with drug resistance t
Evaluation of the management of pretreatment HIV drug resistance by oligonucleotide ligation assay: a randomised controlled trial.
Method: Peripheral blood mononuclear cells were evaluated for NNRTI and NRTI drug resistance mutations, K103N, Y181C, G190A, and K65R, and M184V, using a quantitative OLA.
Method: We evaluated K65R in all pre-ART specimens retrospectively as tenofovir became more available concomitantly with efavirenz.
Pretreatment HIV drug resistance spread within transmission clusters in Mexico City.
PMID: 31819984
2020
The Journal of antimicrobial chemotherapy
Result: K65R was very rare and only observed as a low-frequency variant.
Discussion: Moreover, consistent with previous observations, DRMs with low transmission fitness due to high replicative impairment such as M184V/I and K65R were not shared within the Mexican network, and were observed mostly at low within-host frequencies in the study population.
Trend of HIV-1 drug resistance in China: A systematic review and meta-analysis of data accumulated over 17 years (2001-2017).
Result: The percentages of four mutations K65R (NRTI), M184I/V (NRTI), G190A/S (NNRTI) and M230L (NNRTI) were significantly correlated with subtypes (p < 0.05) (Table 2).
Discussion: Third, two NRTI (M184V/I and K65R), three NNRTI (K103N/S, Y181C/I and G190A/S) and one PI (M46I/L) mutations had higher prevalence than other SDRMs in China.
Pre-treatment HIV-drug resistance associated with virologic outcome of first-line NNRTI-antiretroviral therapy: A cohort study in Kenya.
Result: Among the 59 participants with PDR, NNRTI mutations (K103N, Y181C, G190A) were detected in all but one (98 3%) and NRTI mutations (K65R, M184V) were detected in 14 (23 7%).
Table: K65R
Discussion: An economical and easy to use point of care OLA kit that assesses NNRTI mutations K103N, Y181C, G190A and NRTI mutations M184V and K65R could be used in resource-limited settings to test patients prior to starting EFV-based ART, at virologic failure
Comparison of HIV drug resistance profiles across HIV-1 subtypes A and D for patients receiving a tenofovir-based and zidovudine-based first line regimens in Uganda.
Abstract: As expected, discriminatory DRMs such as K65R, L74I, and Y115F were noted in Tenofovir (TDF) containing regimens while the Thymidine Analogue Mutations (TAMs) L210W and T215 mutations were in Zidovudine (AZT)-based regimens.
Abstract: The most prevalent Nucleoside Reverse Transcriptase Inhibitor (NRTI) mutations were M184V/I (67.3%), K219/Q/E (22.6%) and K65R (21.1%).
Discussion: K65R is highly selected for in TDF containing regimens.
Discussion: TAMs and K65R had a prevalence of 35.7% and 23.1% respectively, similar to a study done in Uganda where K65R mutation had a prevale
HIV-1 subtypes and drug resistance mutations among female sex workers varied in different cities and regions of the Democratic Republic of Congo.
Result: Other NRTI resistance mutations were K65R and K219R.
Table: K65R
Discussion: Moreover, we also observed the K65R mutation, which can compromise the efficacy of d4T in first-line regimens and ABC and ddI in second-line regimens in the DRC.
The S68G polymorphism is a compensatory mutation associated with the drug resistance mutation K65R in CRF01_AE strains.
Figure: 302335, 301844, 301770, and 301635 were four patients infected with CRF01_AE, in whom both the S68G and K65R mutations were detected after treatment failure.
Figure: K65R/S68G: 4:6 (a), 1:1 (b), and 9:1 (c).
Figure: Black columns and white columns represent the K65R/S68G double mutation and K65R mutation pseudoviruses with mutant pol gene sequences obtained from patients, respectively.
Figure: Growth competition assay for the K65R and K65R/S68G infectious clones.
Figure: Temporal association of S68G and K65R muta
Characteristics of drug resistance in HIV-1 CRF55_01B from ART-experienced patients in Guangdong, China.
PMID: 32300784
2020
The Journal of antimicrobial chemotherapy
Abstract: Among DRMs, M184V (43.83%) was the most frequent NRTI DRM, followed by K65R (23.46%), and V179E (98.77%) was the most frequent NNRTI DRM, followed by K103N (47.53%) and Y181C (14.81%).
Drug Resistance Mutations Against Protease, Reverse Transcriptase and Integrase Inhibitors in People Living With HIV-1 Receiving Boosted Protease Inhibitors in South Africa.
Result: The K65R/N mutation occurred in five (5%) patients; K65R occurred in two (2%) patients receiving AZT plus 3TC, and in one (1%) patient receiving TDF plus FTC.
Table: K65R/N
Discussion: In our study, M184V, L74V, K65R, and Y115F were the most common major NRTI RAMs in patients receiving LPV/r as their bPIs.
Discussion: The K65R and Y115F RAMs occurred more frequently in patients receiving AZT plus 3TC, rather than in patients receiving ABC plus 3TC.
HIV mutation literature information.
PMID: 
2020
The Journal of infectious diseases
Browser Board
Co-occurred Entities
Filtrator
ViMRT