Conclusion: We have previously found that three other reversion mutations (V247I and T242N in Gag as well as I64T in Tat) also did not cause detectable fitness differences.
Reversion and T cell escape mutations compensate the fitness loss of a CD8+ T cell escape mutant in their cognate transmitted/founder virus.
Abstract: Two reversion mutations, Figure: (A) Frequencies of the I64T mutation in the Tat protein at different time points (days post Fiebig stage I/II).
Figure: Amino acid substitutions at the I64T mutation site were highlighted in red.
Figure: No fitness costs of the early reversion mutation I64T in Tat/Rev overlapping region.
Figure: Relative fitness of the I64T mutant was determined by comparing to the cognate T/F virus in the single-passage assay (B) and the multiple-passage assay (C).
Discussion: It took months for both the V247I and I64T reversion mutations to become fixed in the population from the first time of detection.
Impact of immune escape mutations on HIV-1 fitness in the context of the cognate transmitted/founder genome.
Method: The tat/env fragment was amplified by using the PCR primers R-lower: 5' Acry-GGAAGCACCCAGGAAGTCAGC-3' (nt 5862-5882) and R-upper: 5'-GTATCCTCTGATGGGAGGGGCATA-3' (nt 7527-7550), and the amplicons were annealed with the sequencing primer Rev7: 5'-ATGCTACTTACTGCTTTGGTAGAGGCGCTTGATTA-3' (nt 6022-6056) to detect the I64T mutation or the sequencing primer Rev13: 5'-CCTCCTGAGGAATGGTTAAAGACTAT-3' (nt 7299-7324) to detect the R355K mutation.
Figure: (C) Frequencies of the viruses with I64T and/or R355K mutations at screening and later time points were determined by SGA .
Figure: Two recombinants (viruses with I64T or R355K mutation)
HIV mutation literature information.
PMID: 
2017
Retrovirology
Browser Board
Co-occurred Entities
Filtrator
ViMRT