Result: To establish their contribution to MxB sensitivity we made the HIV-1 R9 GFP quadruple mutant V86T, H87Q, A92P M96I (named 'TQPI') to mimic the 93BR020 sequence in the CypA binding loop in CA and measured TQPI sensitivity to inhibition by CsA and MxB (Figure 4D).
Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes.
Result: Of interest, we observed that the capsid mutants containing the N74D change, either alone or in combination with the V86M and/or H87Q capsid changes, exhibited increased infectivity (4.9- to 13.9-fold increase) in the marmoset B-LCLs compared to WT viruses.
Result: The V86M and H87Q capsid mutants also exhibited increased infectivity in the marmoset cells compared to WT viruses, although the escape was not as efficient as that of the N74D mutant.
Discussion: In our study, we found that the N74D and, to a lesser degree, the V86M and H87Q
Silent mutations at codons 65 and 66 in reverse transcriptase alleviate indel formation and restore fitness in subtype B HIV-1 containing D67N and K70R drug resistance mutations.
Result: In addition, part of the capsid coding region was sequenced, which confirmed the absence of the H87Q mutation reported to confer a replication advantage to HIV-1 in cyclophilin A-rich cells such as MT-2.
Tissue-specific restriction of cyclophilin A-independent HIV-1- and SIV-derived lentiviral vectors.
Abstract: Here, the effect of lentiviral CypA dependence on restriction in different tissues was examined by engineering an HIV-1 capsid quadruple mutant (V(86)P/H(87)Q/I(91)V/M(96)I) lentiviral vector (HIV(quad)) that is CypA-independent.
Analysis of near full-length genomic sequences of drug-resistant HIV-1 spreading among therapy-naive individuals in Nagoya, Japan: amino acid mutations associated with viral replication activity.
PMID: 18620491
2008
AIDS research and human retroviruses
Abstract: All 12 viruses conserved both an H87Q mutation in the cyclophilin A-binding site of Gag p24 (capsid) and a T23N mutation in the cysteine-rich domain of Tat protein.
Naturally occurring capsid substitutions render HIV-1 cyclophilin A independent in human cells and TRIM-cyclophilin-resistant in Owl monkey cells.
PMID: 16199531
2005
The Journal of biological chemistry
Abstract: Sequencing analyses revealed that these viruses encode capsid substitutions within the CypA-binding site (V86P/H87Q/I91V/M96I).
Influence of gag on human immunodeficiency virus type 1 species-specific tropism.
Abstract: We also show that sensitivity to restriction is controlled by an H87Q mutation in the capsid, implicated in the immune control of HIV-1, possibly linking immune and innate control of HIV-1 infection.
HIV mutation literature information.
PMID: 
2020
eLife
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