Discussion: It has also been reported that the hyper-stable E45A CA mutant can still partially open.
Discussion: These results are in line with previous studies showing that hyper-stable mutants E45A, Q63A/Q67A, 5Mut (Q67H/K70R/H87P/T107N/L111) and A14C/E45C can reverse transcribe and with a recent report saying that reverse transcription can complete in whole WT cores.
Characterization of HIV-1 uncoating in human microglial cell lines.
Discussion: The N74D, E45A, and A92E mutants were chosen because they significantly altered the rate of uncoating in OMK cells in our previous study.
Discussion: The R132T mutation may restore an interaction surface needed for uncoating, and disruption of this region by the E45A mutation would result in delayed uncoating.
Discussion: The E45A mutation produces a hyperstable capsid lattice with increased stiffness in atomic force microscopy assays.
Discussion: showed that the R132T mutation restored the ability of E45A virus to bind the drug PF74.
Multiple Pathways To Avoid Beta Interferon Sensitivity of HIV-1 by Mutations in Capsid.
Discussion: For instance, the N74D and E45A mutations appear to directly decrease the rate of uncoating without any impact on the kinetics of reverse transcription.
PF74 Reinforces the HIV-1 Capsid To Impair Reverse Transcription-Induced Uncoating.
Abstract: At a PF74 concentration of 10 muM, the mechanical stability of the core is increased to a level similar to that of the intrinsically hyperstable capsid mutant E45A.
Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport.
Method: The pR9DeltaEnv vector containing the WT CA or CA mutations E45A, and K203A was described previously.
Result: 3H) cores made of the hyper-stable E45A CA mutant accumulated in the cytoplasm to a greater extent than WT/CA cores, whereas there were rare surviving IN complexes after the quick uncoating of the unstable K203A CA mutant.
Result: In spite of its marked accumulation around the NE, nuclear entry of the E45A mutant was dramatically reduced compared to WT cores.
Discussion: However, the infectivity defect conferred by the
An HIV-1 capsid binding protein TRIM11 accelerates viral uncoating.
Result: E45A mutant has been reported to increase the capsid stability, N74D resides in a conserved hydrophobic binding pocket, while P90A, G89V and G94V reside in the CypA-binding loop.
Result: Similar to wild type HIV-1, the reverse transcription levels of E45A and N74D were restricted in TRIM11-HA expressing cells while enhanced in TRIM11 knockdown cells.
HIV-1 capsid is involved in post-nuclear entry steps.
Abstract: However, the hardening effect of disulfide crosslinking (A204C and A14C/E45C) is lower than that of hydrophobic interactions (E45A and E45A/R132T).
Abstract: We find that hyperstable CA mutant assemblies (A204C, A14C/E45C, E45A and E45A/R132T) are significantly stiffer than WT assemblies.
Introduction: Comparing the wild type with these hyperstable mutants provides insight into the stabilizing effect of disulfide bonds (A204C and A14C/
Identification of capsid mutations that alter the rate of HIV-1 uncoating in infected cells.
Abstract: We found that p24(CA) mutations can significantly increase (A92E), delay (E45A and N74D), or have no effect (G94D) on the rate of uncoating and that these alterations are not due to changes in reverse transcription.
Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay.
Abstract: Analysis of the E45A CA mutant suggests that initial core opening is independent of complete capsid disassembly.
Introduction: Surprisingly, the previously identified E45A CA mutant, which exhibits hyperstable cores in vitro, exhibited efficient staining of viral-associated RNA through cores in vitro and accelerated staining kinetics in vivo, suggestive of a permeable capsid.
Method: The pNLdVdE-luc plasmid had the following CA amino acid substitutions introduced by via cloning from other HIV-1NL4-3 vectors or PCR mutagenesis: E45A, E45A/R132T, K203A, or 5Mut.|mg
HIV mutation literature information.
PMID: 
2021
PLoS pathogens
Browser Board
Co-occurred Entities
Filtrator
ViMRT