Result: To further investigate the activity of RT in the E300R virions, an endogenous reverse transcription (ERT) assay was performed with similar amounts of WT and E300R virions incubated with deoxynucleoside triphosphates (dNTPs).
Figure: Data are the mean number of relative BRET
Figure: ERT assays were performed with similar amounts of WT and E300R HIV-1 virions incubated with or without dNTPs for 6 h, and qPCR was used to measure ssDNA (D) and first strand transfer DNA (E).
Discussion: Although the pattern is the same between cell types, the greater impact of the E300R mutation on virus replication in Jurkat and CD4+ T cells than in TZM-bl cells may result from a cell type difference, as WT virus replication was weak compared to that in Jurkat and CD4+ T cells.
HIV mutation literature information.
PMID: 
2018
mBio
Browser Board
Co-occurred Entities
Filtrator