Result: The time when half of the viral RTCs had completed reverse transcription was approximately 2 h later for HIV-1 with the E300R mutation (50% delay), 4 h later for HIV-1 with the E298R mutation (100% delay), and 30 min later for E297R (14% delay) than for WT HIV-1.

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Discussion: The reduced efficiency of first strand transfer in HIV-1 with the E298R mutation indicates a partially defective RT enzyme compared to WT

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Discussion: Unexpectedly, the mild increase in predicted structural instability of the E297R mutation in RT did not reduce RT catalytic activity and instead significantly increased RT catalytic activity by 50% in vitro.

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