Result: After a median antepartum zidovudine duration of 10.4 weeks in both groups and an additional 4 weeks postpartum zidovudine-plus-didanosine in PHPT-4, new NRTI resistance mutations were detected by consensus sequencing in five PHPT-4 subjects (2.3%; four K70R and one D67N+K70R) and two PHPT-2 controls (0.9%; one D67N, one K70R) (Table 4).
Table: D67N
N348I in reverse transcriptase provides a genetic pathway for HIV-1 to select thymidine analogue mutations and mutations antagonistic to thymidine analogue mutations.
Result: Previous biochemical studies suggested that this phenotype was due to the Y181C mutation decreasing the AZT-MP excision activity of both WT and D67N/K70R/T215F/K219Q HIV-1 RT by directly impacting ATP binding and/or the rate of AZT-MP excision.
[Evolution of HIV-1 drug resistance in patients failing combination antiretroviral therapy].
Abstract: K103N, G190A, Y181C, K101P, M184V, D67N, K70R, T215Y and K219 were most common mutations.
Constrained patterns of covariation and clustering of HIV-1 non-nucleoside reverse transcriptase inhibitor resistance mutations.
PMID: 20462946
2010
The Journal of antimicrobial chemotherapy
Method: We also examined the extent to which the 52 NNRTI-selected mutations covaried with mutations at 12 major nucleoside reverse transcriptase inhibitor (NRTI) resistance positions, including M41L, K65R, D67N, T69S_SS, K70E, K70R, L74V, L74I, V75M/T, Y115F, Q151M, M184V/I, L210W, T215F and T215Y.
Result: The NNRTI
Prevalence and clinical significance of HIV drug resistance mutations by ultra-deep sequencing in antiretroviral-naive subjects in the CASTLE study.
Drug resistance is widespread among children who receive long-term antiretroviral treatment at a rural Tanzanian hospital.
PMID: 20576637
2010
The Journal of antimicrobial chemotherapy
Table: D67N
Genetic characterization of HIV type 1 among patients with suspected immune reconstitution inflammatory syndrome after initiation of antiretroviral therapy in Kenya.
PMID: 20624074
2010
AIDS research and human retroviruses
Abstract: These included nucleoside reverse transcriptase inhibitor (RTI) mutations: M41L, K65R, D67N, K70R, M184V, and K219Q, and nonnucleoside RTI mutations: K101P, L100I, K103N, and Y181C.
HIV type 1 pol gene diversity and genotypic antiretroviral drug resistance mutations in Malabo, Equatorial Guinea.
PMID: 20718620
2010
AIDS research and human retroviruses
Abstract: Analysis of antiretroviral drug resistance mutations revealed two patients each harboring one major mutation, M46I in protease and D67N in reverse transcriptase sequences, respectively.
Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART).
PMID: 20818500
2010
International journal of molecular medicine
Abstract: The main mutations associated with nucleoside reverse transcriptase inhibitor (NRTI) resistance were M184V in 82 (64.6%), and the thymidine analog mutations were D67N in 51 (40.1%) tests, K70R in 45 (35.4%), T215Y in 40 (31.5%), and M41L in 38 (30.0%).
Structural basis of HIV-1 resistance to AZT by excision.
Abstract: We determined five crystal structures: wild-type reverse transcriptase-double-stranded DNA (RT-dsDNA)-AZTppppA; AZT-resistant (AZTr; M41L D67N K70R T215Y K219Q) RT-dsDNA-AZTppppA; AZTr RT-dsDNA terminated with AZT at dNTP- and primer-binding sites; and AZTr apo reverse transcriptase.
Introduction: The mutations commonly associated with excision-mediated AZT resistance are M41L, D67N, K70R, L210W, T215Y,
ViMRT
HIV mutation literature information.
PMID: 
2010
Clinical infectious diseases
