Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase.
Result: The infection of the class I mutant D64E virus resulted only in a basal level of luc activity that was approximately 104-fold lower than the wild type.
Result: These result
Result: To test whether the IN catalytic mutants could induce the lethal phenotype in HP16 yeast strain, we first introduced the class I IN mutants D64E, D116A and the double mutant (D64E/D116A) into yeast strain HP16 and determined their effect on yeast growth.
Result: Total viral DNA synthesis during infections with the V165A-, A179P- and KR186,7AA-containing viruses was similar to that following infection with the wild type and D64E mutant viruses.
Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import.
Abstract: Analysis of their effects on viral infection in a VSV-G pseudotyped RT/IN trans-complemented HIV-1 single cycle replication system revealed that all three C-terminal mutant viruses
Result: Results showed that the number of infected cells (beta-Gal positive cells) for D64E mutant reached approximately 14% of the wild type level (data not shown).
Discussion: Also, in our infection experiments, a specific integration-defective class I mutant D64E virus was introduced in order to monitor the viral gene expression from unintegrated HIV-1 DNA species that are already translocated into nucleus during virus infection.
Discussion: Consistent with a previous study, infection with D64E mutant virus did not affect reverse transcription as compared to wt virus infection.
HIV mutation literature information.
PMID: 
2010
Virology journal
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