Result: Mutations Q16R, Q181R and W182R did not substantially modify the total HBsAg production pattern in M88 and P38.II plasmids (Figure 4).
Result: Seven OBI-specific substitutions introducing an arginine residue in S TMDs were investigated: Q16R in TMD1, C85R, L87R, L88R, and C90R in TMD2, and Q181R and W182R in TMD3.
Discussion: The substitutions Q181R and W182R had no significant impact on HBsAg production pa