Abstract: By co-variation analysis, correlations were observed for R122P+S167L (phi=0.68, P=0.01), T116N+S143L (phi=0.53, P=0.03), and Y100S+S143L (phi=0.67, p<0.001).
Abstract: Mutants (obtained by site-directed mutagenesis) carrying T116N, T116N+S143L, R122P, R122P+Q101R, or R122P+S167L strongly decreased HBsAg-reactivity (54.9+-22.6S/CO, 31.2+-12.0S/CO, 6.1+-2.4S/CO, 3.0+-1.0S/CO and 3.9+-1.3S/CO, respectively) compared to wild-type (306.8+-64.1S/CO).